ZFIN ID: ZDB-PUB-030312-1
Expression of the zebrafish recognition molecule F3/F11/contactin in a subset of differentiating neurons is regulated by cofactors associated with LIM domains
Gimnopoulos, D., Becker, C.G., Ostendorff, H.P., Bach, I., Schachner, M., and Becker, T.
Date: 2002
Source: Mechanisms of Development   119: S135-S141 (Journal)
Registered Authors: Becker, Catherina G., Becker, Thomas, Schachner, Melitta
Keywords: none
MeSH Terms:
  • Animals
  • Axons/metabolism
  • Contactins*
  • Homeodomain Proteins/metabolism
  • Molecular Sequence Data
  • Neurons/metabolism
  • Transcription Factors/metabolism
  • Zebrafish*/genetics
PubMed: 14516675 Full text @ Mech. Dev.
We have identified a zebrafish homolog of the F3/F11/contactin (F3) recognition molecule. The gene shares 55% amino acid identity with F3 molecules in other vertebrates. Expression of F3 mRNA is first detectable at 16h post-fertilization (hpf) in trigeminal and Rohon-Beard neurons. At 18-24hpf, additional weaker expression is present in discrete cell clusters in the hindbrain, in the anterior lateral line/ acoustic ganglion and in spinal motor neurons. Transcription factors of the LIM homeodomain class (LIM-HD) and their associated cofactors CLIM/ NLI/Ldb (CLIM) have been implicated in the development of peripheral axons of trigeminal and Rohon-Beard neurons. We demonstrate that ectopic overexpression of a dominant-negative CLIM molecule early during zebrafish development strongly reduces expression of F3 mRNA in these neurons indicating regulation of F3 by the LIM-HD protein network. These results and the spatiotemporal correlation of F3 expression with axonal differentiation in a subset of primary neurons suggest an important role of F3 for axon growth.