PUBLICATION
Myostatin precursor is present in several tissues in teleost fish: a comparative immunolocalization study
- Authors
- Radaelli, G., Rowlerson, A., Mascarello, F., Patruno, M., and Funkenstein, B.
- ID
- ZDB-PUB-030304-10
- Date
- 2003
- Source
- Cell and tissue research 3119(2): 239-250 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Aging/physiology*
- Animals
- Immunohistochemistry
- Larva/cytology
- Larva/metabolism
- Myostatin
- Organ Specificity
- Protein Precursors/metabolism*
- Sea Bream/growth & development
- Species Specificity
- Transforming Growth Factor beta/metabolism*
- Zebrafish/growth & development
- Zebrafish Proteins
- PubMed
- 12596043 Full text @ Cell Tissue Res.
Citation
Radaelli, G., Rowlerson, A., Mascarello, F., Patruno, M., and Funkenstein, B. (2003) Myostatin precursor is present in several tissues in teleost fish: a comparative immunolocalization study. Cell and tissue research. 3119(2):239-250.
Abstract
In this study, the distribution of myostatin was investigated during larval and postlarval developmental stages of Sparus aurata(sea bream), Solea solea(sole) and Brachydanio rerio(zebrafish) by immunohistochemistry using antisera raised against a synthetic peptide located within the precursor region of sea bream myostatin. All the three species examined showed the strongest immunoreactivity in red skeletal muscle in juveniles and adults. During larval development of sea bream, strong staining was detected in skin and brain. Immunoreactivity was also found in muscle, pharynx, gills, pancreas and liver. From metamorphosis, immunoreactivity was identifiable in the oesophagus, in the apical portion of the stomach epithelium, in the intestinal epithelium and in renal tubules. In larval zebrafish at hatching, the most intense myostatin immunoreactivity was evident in the skin epithelium. Immunoreactivity was also found in the retina and brain. In the adult, an intense immunostaining occurred in the gastrointestinal tract as well as in the ovary. In sole larvae, immunoreactivity was found in liver and intestine. Our results support the hypothesis suggested earlier that myostatins in fish have retained a different partition (compared with mammals) of the expression patterns and functions which characterized the ancestral gene before the duplication event that gave rise to growth differentiation factor-11 (GDF-11) and GDF-8 (myostatin).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping