PUBLICATION
In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina
- Authors
- Das, T., Payer, B., Cayouette, M., and Harris, W.A.
- ID
- ZDB-PUB-030219-1
- Date
- 2003
- Source
- Neuron 37(4): 597-609 (Journal)
- Registered Authors
- Harris, William A.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Division/physiology
- Fluorescent Dyes
- Luminescent Proteins/genetics
- Microscopy, Video
- Mitosis
- Mutation
- Neurons/cytology*
- Rats
- Recombinant Fusion Proteins/genetics
- Retina/cytology*
- Retina/embryology*
- Stem Cells/cytology
- Time Factors
- Zebrafish
- PubMed
- 12597858 Full text @ Neuron
Citation
Das, T., Payer, B., Cayouette, M., and Harris, W.A. (2003) In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina. Neuron. 37(4):597-609.
Abstract
Asymmetric cell division has been proposed to be a mechanism for generating cell fate diversity. Evidence in both vertebrates and invertebrates has suggested that division of neuroblasts along the apico-basal axis allows for unequal segregation of cell fate determinants and consequently distinct cell fates for the resulting
daughter cells. Here, Das et al. used in vivo two-photon imaging to reconstruct cell divisions in the zebrafish retina and show that, surprisingly, there are no apico-basal cell divisions in the zebrafish retina during the period of neurogenesis. Rather, the period of neurogenesis correlates with an unexpected shift in orientation of cell division from central-peripheral to circumferential. Further, in mutants that exhibit delayed timing of neurogenesis and differentiation, this shift is also delayed, suggesting a role for this shift in initiating neurogenesis in the zebrafish retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping