PUBLICATION

Pituitary corticotroph ontogeny and regulation in transgenic zebrafish

Authors
Liu, N.A., Huang, H., Yang, Z., Herzog, W., Hammerschmidt, M., Lin, S., and Melmed, S.
ID
ZDB-PUB-030211-9
Date
2003
Source
Molecular endocrinology (Baltimore, Md.)   17(5): 959-966 (Journal)
Registered Authors
Hammerschmidt, Matthias, Herzog, Wiebke, Huang, Haigen, Lin, Shuo, Liu, Ningai, Yang, Zhongan
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cell Lineage
  • Conserved Sequence
  • Dexamethasone/pharmacology
  • Down-Regulation/drug effects
  • Embryo, Nonmammalian/drug effects
  • Female
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Male
  • Molecular Sequence Data
  • Pituitary Gland/cytology
  • Pituitary Gland/drug effects
  • Pituitary Gland/embryology
  • Pituitary Gland/physiology*
  • Pro-Opiomelanocortin/genetics*
  • Pro-Opiomelanocortin/metabolism
  • Promoter Regions, Genetic
  • Vertebrates/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
12576489 Full text @ Mol. Endocrinol.
Abstract
We characterized zebrafish pro-opiomelanocortin (POMC) gene promoter, and sequence analysis revealed that the promoter contains regulatory elements conserved among vertebrate species. To monitor the ontogeny of the pituitary POMC lineage in living vertebrates, we generated transgenic zebrafish expressing green fluorescent protein (GFP) driven by the POMC promoter. Zebrafish POMC-GFP is first expressed asymmetrically as two bilateral groups of cells most anterior to the neural ridge midline at 18-20 h post fertilization (hpf). POMC-GFP positive cells then fuse into a single cell mass within the pituitary anlage after 24 hpf, and subsequently organize as distinct anterior and posterior domains between 48 to 64 hpf. Immunohistochemical studies with ACTH and alphaMSH antisera showed that POMC-GFP was mainly targeted to both anterior and posterior pituitary corticotrophs, whereas posterior pituitary region melanotrophs did not express GFP. To determine in vivo zebrafish corticotroph responses, dexamethasone (10(\-5) M) was added to live embryos, which selectively suppressed POMC-GFP expression in the anterior group of corticotrophs, suggesting a distinct domain responsive to glucocorticoid feedback. Transgenic zebrafish with specific POMC-GFP expression in pituitary corticotrophs offers a powerful genetic system for in vivo study of vertebrate corticotroph lineage development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes