ZFIN ID: ZDB-PUB-030211-5
cDNA sequences of the authentic keratins 8 and 18 in zebrafish
Schaffeld, M., Knappe, M., Hunzinger, C., and Markl, J.
Date: 2003
Source: Differentiation; research in biological diversity   71(1): 73-82 (Journal)
Registered Authors:
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Humans
  • Keratin-18
  • Keratin-8
  • Keratins/chemistry*
  • Keratins/genetics
  • Keratins/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Tissue Distribution
  • Zebrafish
  • Zebrafish Proteins/chemistry*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 12558605 Full text @ Differentiation
From the zebrafish Danio rerio, we have cDNA cloned and sequenced a novel type II and a novel type I keratin, termed DreK8 and DreK18, respectively. We identified DreK8/18 as the true orthologs of the human keratin pair K8/18 as follows: (i) MALDI-MS assignment to the biochemically identified K8 and K18 candidates that are co-expressed in simple epithelia and absent in epidermal keratinocytes; (ii) multiple sequence alignments and phylogenetic tree analysis, showing that DreK8, within the phylogenetic tree of type II keratins, forms a highly bootstrap-supported branch together with K8 from goldfish and rainbow trout, whereas DreK18, within the phylogenetic tree of type I keratins, groups with the K18 sequences from all other vertebrates studied; (iii) presence of a conserved motif in the tail domain of DreK8 ( VxKxxETxDGxxVSESSxV) that is typical for all hitherto sequenced K8 orthologs. Moreover, several zebrafish type II keratin sequences published by other authors have now been assigned to epidermal keratins , previously identified biochemically.