ZFIN ID: ZDB-PUB-030115-20
Analysis of gene function in the zebrafish retina
Malicki, J., Jo, H., Wei, X., Hsiung, M., and Pujic, Z.
Date: 2002
Source: Methods (San Diego, Calif.)   28(4): 427-438 (Journal)
Registered Authors: Jo, Hakryul, Malicki, Jarema, Pujic, Zac, Wei, Xiangyun
Keywords: Zebrafish; retina; Morpholino; Overexpression; Knockdown; Eye; Development; Neuronal; Heat shock; Transgenic
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Gene Expression Regulation, Developmental/genetics*
  • Molecular Biology/methods*
  • Molecular Sequence Data
  • Mutagenesis
  • Retina/growth & development*
  • Retina/physiology*
  • Zebrafish
PubMed: 12507461 Full text @ Methods
Mutagenesis screens in zebrafish have uncovered several hundred mutant alleles affecting the development of the retina and established the zebrafish as one of the leading models of vertebrate eye development. In addition to forward genetic mutagenesis approaches, gene function in the zebrafish embryo is being studied using several reverse genetic techniques. Some of these rely on the overexpression of a gene product, others take advantage of antisense oligonucleotides to block function of selected loci. Here we describe these methods in the context of the developing eye.