PUBLICATION
dsRNA Mediated masculanization in the zebrafish Danio rerio
- Authors
- Patil, J.G., Grewe, P.M., Deagle, B.E., Gurney, R.H., and Thresher, R.E.
- ID
- ZDB-PUB-030103-13
- Date
- 2001
- Source
- 6th Asian Fisheries Forum Book of Abstracts : p. 193 (Abstract)
- Registered Authors
- Grewe, Peter, Patil, Jawahar G.
- Keywords
- Aromatase; Genetic engineering; Double-stranded RNA; transgenic fish; masculanization; DNA; Biotechnology; Monosex culture; Aquaculture techniques; Sex determination; Danio rerio
- MeSH Terms
- none
- PubMed
- none
Citation
Patil, J.G., Grewe, P.M., Deagle, B.E., Gurney, R.H., and Thresher, R.E. (2001) dsRNA Mediated masculanization in the zebrafish Danio rerio. 6th Asian Fisheries Forum Book of Abstracts. :p. 193.
Abstract
It has been well recognized that there is a great deal of economic advantage in farming monosex populations of fish largely favouring the sex that shows the fastest growth (eg males of cichlids and females of Salmon). Monosex populations of both males and females have been routinely achieved by external administration of appropriate sex steroids in combination with breeding techniques. However there seem to be concerns associated with use of steroids in fish destined for human consumption as well as environmental influences on sex differentiation such as temperature and inhibitors of aromatase, a key steroidogenic enzyme on the phenotypic/physiological sex of fish. In order to address some of these limitations as well as elucidate the role of aromatase in sex differentiation, we have begun to characterize and transgenically modify the expression of aromatase gene in the zebrafish. Here we report our preliminary data that suggests that PGC specific expression of aromatase dsRNA results in masculanization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping