PUBLICATION
Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and Mammalian cell proliferation
- Authors
- Lerch-Gaggl, A., Haque, J., Li, J., Ning, G., Traktman, P., and Duncan, S.A.
- ID
- ZDB-PUB-021119-3
- Date
- 2002
- Source
- The Journal of biological chemistry 277(47): 45347-45355 (Journal)
- Registered Authors
- Duncan, Stephen A.
- Keywords
- none
- MeSH Terms
-
- 3T3 Cells
- Animals
- Cell Division/physiology*
- Cell Nucleolus/metabolism*
- Cell Nucleus/metabolism
- Cells, Cultured
- Embryo, Mammalian/physiology
- Embryo, Mammalian/ultrastructure
- Embryo, Nonmammalian
- Female
- Gene Targeting
- Genotype
- Liver/cytology
- Liver/metabolism
- Mice
- Microscopy, Confocal
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism
- Phenotype
- Proteins/metabolism*
- Ribosomes/metabolism*
- Tissue Distribution
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins*
- PubMed
- 12237316 Full text @ J. Biol. Chem.
Citation
Lerch-Gaggl, A., Haque, J., Li, J., Ning, G., Traktman, P., and Duncan, S.A. (2002) Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and Mammalian cell proliferation. The Journal of biological chemistry. 277(47):45347-45355.
Abstract
Mutation of the zebrafish pescadillo gene blocks expansion of a number of tissues in the developing embryo, suggesting roles for its gene product in controlling cell proliferation. We report that levels of the pescadillo protein increase in rodent hepatocytes as they enter the cell cycle. Pescadillo protein localizes to distinct substructures of the interphase nucleus including nucleoli, the site of ribosome biogenesis. During mitosis pescadillo closely associates with the periphery of metaphase chromosomes and by late anaphase is associated with nucleolus- derived foci and prenucleolar bodies. Blastomeres in mouse embryos lacking pescadillo arrest at morula stages of development, the nucleoli fail to differentiate and accumulation of ribosomes is inhibited. We propose that in mammalian cells pescadillo is essential for ribosome biogenesis and nucleologenesis and that disruption to its function results in cell cycle arrest.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping