ZFIN ID: ZDB-PUB-021106-1
Inhibitory interactions controlling organizer activity in fish
Peyriéras, N., Lu, Y., Renucci, A., Lemarchandel, V., and Rosa, F.
Date: 1996
Source: Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie   319(12): 1107-1112 (Journal)
Registered Authors: Lemarchandel, Valerie, Renucci, Armand, Rosa, Frederic
Keywords: none
MeSH Terms:
  • Animals
  • Mesoderm/cytology
  • Mesoderm/drug effects
  • Mutation
  • Nucleolus Organizer Region*
  • RNA, Messenger/genetics
  • RNA, Messenger/pharmacology
  • Transforming Growth Factor beta/genetics
  • Zebrafish/embryology*
PubMed: 9091181
An experimental system allowing the observation of 2 active organizing centers during zebrafish development is described. It was achieved by injection into a single marginal cell at the 16-cell stage of TARAM-A-D mRNA. TARAM-A-D was previously described as the mutated constitutive form of a type I receptor for transforming growth factor beta. In 80% of the injected embryos, 2 distinct organizers were observed at the onset of gastrulation. At the end of gastrulation, these embryos showed duplicated axial structures. Nevertheless, only 25% of the injected embryos displayed a recognizable axis duplication after 1 day of development. This paradox is taken as an evidence for suppressive effects exerted in a reciprocal manner when more than 1 organizing center is present.