A mutagenesis screen to identify maternal factors required in early zebrafish development
- Wagner, D.S., Dosch, R., Holloway, B.A., Mei, W.Y., Mintzer, K.A., and Mullins, M.C.
- Developmental Biology 247(2): 523 (Abstract)
- Registered Authors
- Dosch, Roland, Holloway, Beth A., Mei, Wenyan, Mintzer, Keith A., Mullins, Mary C., Wagner, Daniel
- MeSH Terms
Wagner, D.S., Dosch, R., Holloway, B.A., Mei, W.Y., Mintzer, K.A., and Mullins, M.C. (2002) A mutagenesis screen to identify maternal factors required in early zebrafish development. Developmental Biology. 247(2):523.
We have conducted a four-generation maternal-effect screen to identify maternally supplied factors that are critical for oogenesis and embryogenesis in the zebrafish. We have screened 600 mutagenized genomes and have identified approximately 75 maternal-effect mutants. The mutants we recovered affect a wide variety of developmental processes, including egg synthesis, egg activation, cell division, cell survival, embryonic pattern formation, and morphogenesis. The spectrum of phenotypes we recovered clearly demonstrates a complex maternal contribution to early embryo development in the zebrafish. Maternally supplied factors are not only required for early events such as egg activation and early cell division but also at later periods where maternally supplied factors persist and coordinate with newly synthesized zygotic factors to execute a normal developmental program.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes