ZFIN ID: ZDB-PUB-021016-12
B cells develop in the zebrafish pancreas
Danilova, N. and Steiner, L.A.
Date: 2002
Source: Proceedings of the National Academy of Sciences of the United States of America   99(21): 13711-13716 (Journal)
Registered Authors: Danilova, Nadia, Steiner, Lisa
Keywords: none
MeSH Terms:
  • Animals
  • B-Lymphocytes/cytology
  • B-Lymphocytes/immunology*
  • DNA/genetics
  • Gene Expression Regulation, Developmental
  • Gene Rearrangement, B-Lymphocyte
  • Genes, RAG-1
  • Immunoglobulin mu-Chains/genetics
  • In Situ Hybridization
  • Lymphopoiesis/genetics
  • Lymphopoiesis/immunology
  • Mice
  • Pancreas/embryology*
  • Pancreas/growth & development
  • Pancreas/immunology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/immunology*
PubMed: 12370418 Full text @ Proc. Natl. Acad. Sci. USA
The zebrafish, with its transparent free-living embryo, is a useful organism for investigating early stages in lymphopoiesis. Previously, we showed that T cells differentiate in the thymus by day 4, but no sites for B cell differentiation were seen until 3 weeks. We report here that on day 4, we detect rearrangements of genes encoding B cell receptors in DNA extracted from whole fish. Also by day 4, rag1 transcripts are seen in the pancreas, an organ not previously associated with lymphopoiesis; by day 10, Igmu; transcripts are detected here. Thus, in zebrafish, the pancreas assumes the role of both the liver in fetal mice and the spleen in neonatal mice.