ZFIN ID: ZDB-PUB-020912-1
High-throughput behavioral screening method for detecting auditory response defects in zebrafish
Bang, P., Yelick, P., Malicki, J., and Sewell, W.
Date: 2002
Source: Journal of Neuroscience Methods   118(2): 177-187 (Journal)
Registered Authors: Bang, Pascal I., Malicki, Jarema, Yelick, Pamela C.
Keywords: hearing; auditory system; startle response; hair cell; macula
MeSH Terms:
  • Acoustic Stimulation
  • Air Sacs/physiopathology
  • Animals
  • Hearing/physiology*
  • Image Processing, Computer-Assisted/methods
  • Mutation
  • Reflex, Startle/physiology
  • Spectrum Analysis
  • Zebrafish
PubMed: 12204308 Full text @ J. Neurosci. Methods
We have developed an automated, high-throughput behavioral screening method for detecting hearing defects in zebrafish. Our assay monitors a rapid escape reflex in response to a loud sound. With this approach, 36 adult zebrafish, restrained in visually isolated compartments, can be simultaneously assessed for responsiveness to near-field 400 Hz sinusoidal tone bursts. Automated, objective determinations of responses are achieved with a computer program that obtains images at precise times relative to the acoustic stimulus. Images taken with a CCD video camera before and after stimulus presentation are subtracted to reveal a response to the sound. Up to 108 fish can be screened per hour. Over 6500 fish were tested to validate the reliability of the assay. We found that 1% of these animals displayed hearing deficits. The phenotypes of non-responders were further assessed with radiological analysis for defects in the gross morphology of the auditory system. Nearly all of those showed abnormalities in conductive elements of the auditory system : the swim bladder or Weberian ossicles.