|ZFIN ID: ZDB-PUB-020912-1|
High-throughput behavioral screening method for detecting auditory response defects in zebrafish
Bang, P., Yelick, P., Malicki, J., and Sewell, W.
|Source:||Journal of Neuroscience Methods 118(2): 177-187 (Journal)|
|Registered Authors:||Bang, Pascal I., Malicki, Jarema, Yelick, Pamela C.|
|Keywords:||hearing; auditory system; startle response; hair cell; macula|
|PubMed:||12204308 Full text @ J. Neurosci. Methods|
Bang, P., Yelick, P., Malicki, J., and Sewell, W. (2002) High-throughput behavioral screening method for detecting auditory response defects in zebrafish. Journal of Neuroscience Methods. 118(2):177-187.
ABSTRACTWe have developed an automated, high-throughput behavioral screening method for detecting hearing defects in zebrafish. Our assay monitors a rapid escape reflex in response to a loud sound. With this approach, 36 adult zebrafish, restrained in visually isolated compartments, can be simultaneously assessed for responsiveness to near-field 400 Hz sinusoidal tone bursts. Automated, objective determinations of responses are achieved with a computer program that obtains images at precise times relative to the acoustic stimulus. Images taken with a CCD video camera before and after stimulus presentation are subtracted to reveal a response to the sound. Up to 108 fish can be screened per hour. Over 6500 fish were tested to validate the reliability of the assay. We found that 1% of these animals displayed hearing deficits. The phenotypes of non-responders were further assessed with radiological analysis for defects in the gross morphology of the auditory system. Nearly all of those showed abnormalities in conductive elements of the auditory system : the swim bladder or Weberian ossicles.
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