PUBLICATION
High-throughput behavioral screening method for detecting auditory response defects in zebrafish
- Authors
- Bang, P., Yelick, P., Malicki, J., and Sewell, W.
- ID
- ZDB-PUB-020912-1
- Date
- 2002
- Source
- Journal of Neuroscience Methods 118(2): 177-187 (Journal)
- Registered Authors
- Bang, Pascal I., Malicki, Jarema, Yelick, Pamela C.
- Keywords
- hearing; auditory system; startle response; hair cell; macula
- MeSH Terms
-
- Mutation
- Hearing/physiology*
- Image Processing, Computer-Assisted/methods
- Zebrafish
- Acoustic Stimulation
- Spectrum Analysis
- Reflex, Startle/physiology
- Animals
- Air Sacs/physiopathology
- PubMed
- 12204308 Full text @ J. Neurosci. Methods
Citation
Bang, P., Yelick, P., Malicki, J., and Sewell, W. (2002) High-throughput behavioral screening method for detecting auditory response defects in zebrafish. Journal of Neuroscience Methods. 118(2):177-187.
Abstract
We have developed an automated, high-throughput behavioral screening method for detecting hearing defects in zebrafish. Our assay monitors a rapid escape reflex in response to a loud sound. With this approach, 36 adult zebrafish, restrained in visually isolated compartments, can be simultaneously assessed for responsiveness to near-field 400 Hz sinusoidal tone bursts. Automated, objective determinations of responses are achieved with a computer program that obtains images at precise times relative to the acoustic stimulus. Images taken with a CCD video camera before and after stimulus presentation are subtracted to reveal a response to the sound. Up to 108 fish can be screened per hour. Over 6500 fish were tested to validate the reliability of the assay. We found that 1% of these animals displayed hearing deficits. The phenotypes of non-responders were further assessed with radiological analysis for defects in the gross morphology of the auditory system. Nearly all of those showed abnormalities in conductive elements of the auditory system : the swim bladder or Weberian ossicles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping