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ZFIN ID: ZDB-PUB-020716-22
Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A
Hannus, M., Feiguin, F., Heisenberg, C.-P., and Eaton, S.
Date: 2002
Source: Development (Cambridge, England) 129(14): 3493-3503 (Journal)
Registered Authors: Heisenberg, Carl-Philipp
Keywords: Drosophila; Widerborst; PP2A; zebrafish; planar polarity
MeSH Terms:
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Cadherins/genetics
  • Cell Polarity/genetics
  • Cell Polarity/physiology
  • Drosophila/cytology
  • Drosophila/enzymology*
  • Drosophila/genetics
  • Drosophila/growth & development*
  • Drosophila Proteins/genetics
  • Drosophila Proteins/physiology*
  • Female
  • Frizzled Receptors
  • Gastrula/enzymology
  • Genes, Insect
  • Hair/growth & development
  • Male
  • Membrane Proteins/genetics
  • Microtubules/enzymology
  • Mutation
  • Phenotype
  • Phosphoproteins/genetics
  • Protein Phosphatase 2/genetics
  • Protein Phosphatase 2/physiology*
  • Receptors, G-Protein-Coupled
  • Wings, Animal/cytology
  • Wings, Animal/growth & development
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 12091318
We have identified widerborst (wdb), a B' regulatory subunit of PP2A, as a conserved component of planar cell polarization mechanisms in both Drosophila and in zebrafish. In Drosophila, wdb acts at two steps during planar polarization of wing epithelial cells. It is required to organize tissue polarity proteins into proximal and distal cortical domains, thus determining wing hair orientation. It is also needed to generate the polarized membrane outgrowth that becomes the wing hair. Widerborst activates the catalytic subunit of PP2A and localizes to the distal side of a planar microtubule web that lies at the level of apical cell junctions. This suggests that polarized PP2A activation along the planar microtubule web is important for planar polarization. In zebrafish, two wdb homologs are required for convergent extension during gastrulation, supporting the conjecture that Drosophila planar cell polarization and vertebrate gastrulation movements are regulated by similar mechanisms.