PUBLICATION
Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae
- Authors
- Hill, A.J., Howard, C.V., and Cossins, A.R.
- ID
- ZDB-PUB-020701-6
- Date
- 2002
- Source
- Journal of microscopy 206(3): 179-181 (Journal)
- Registered Authors
- Hill, Adrian
- Keywords
- embedding; fish larvae; resin; stereology
- MeSH Terms
-
- Animals
- Histological Techniques
- Larva/anatomy & histology
- Plastic Embedding/instrumentation
- Plastic Embedding/methods*
- Specimen Handling
- Tissue Embedding/methods
- Zebrafish/anatomy & histology*
- PubMed
- 12067361 Full text @ J. Micros.
Citation
Hill, A.J., Howard, C.V., and Cossins, A.R. (2002) Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae. Journal of microscopy. 206(3):179-181.
Abstract
Stereological sampling regimes, in particular volume and number estimation, often require systematic uniformly random sections throughout a specimen. A method has been developed to increase the efficiency of preparing fish larvae for sectioning prior to histological or stereological analysis. Embedding a group of larvae in a resin block using this technique greatly reduces the quantity of sections produced and allows easy assessment of sample groups. Saving time in this way therefore makes stereology a more viable research tool.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping