PUBLICATION

Atlas of the developing inner ear in zebrafish

Authors
Bever, M.M. and Fekete, D.M.
ID
ZDB-PUB-020403-4
Date
2002
Source
Developmental dynamics : an official publication of the American Association of Anatomists   223(4): 536-543 (Journal)
Registered Authors
Fekete, Donna Marie
Keywords
otic vesicle; macula; saccule; lagena; semicircular canals; membranous labyrinth; transverse canal; endolymphatic duct; Danio; pars inferior; otolith
MeSH Terms
  • Animals
  • Ear, Inner/anatomy & histology*
  • Ear, Inner/embryology
  • Morphogenesis
  • Zebrafish/embryology*
PubMed
11921341 Full text @ Dev. Dyn.
Abstract
This report provides a description of the normal developing inner ear of the zebrafish, Danio rerio, with special focus on the pars inferior. Zebrafish specimens, ranging in age from 3 to 30 days postfertilization (dpf), were processed for standard histologic sections or with a paint-fill method to show three-dimensional morphogenesis of the membranous labyrinth. Adult zebrafish (age 2 years) were also processed for inner ear paint-fills. Although development of the semicircular canals occurs rapidly (by 3 dpf), the pars inferior develops more gradually during days 5-20 postfertilization. A rudimentary endolymphatic duct emerges by 8 dpf. Differentiated hair cells of the lagenar macula are evident by 15 dpf, in a chamber located lateral and posterior to the saccule. By 20 dpf, the saccule itself is separated from the utricle, but remains connected by means of the utriculosaccular foramen. The maculae neglectae, each with differentiated hair cells, lie on the floor of the utricle near this foramen. A medial connection between the sacculi of right and left ears, the transverse canal, is also complete by 20 dpf. A ridge of mesenchyme, previously undescribed, bisects the saccule in zebrafishfry at 20-30 dpf. The images in the paint-fill atlas should provide a baseline for future studies of mutant zebrafish ears.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes