PUBLICATION

her1 and the notch pathway function within the oscillator mechanism that regulates zebrafish somitogenesis

Authors
Holley, S.A., Jülich, D., Rauch, G.J., Geisler, R., and Nüsslein-Volhard, C.
ID
ZDB-PUB-020306-6
Date
2002
Source
Development (Cambridge, England)   129(5): 1175-1183 (Journal)
Registered Authors
Geisler, Robert, Holley, Scott, Jülich, Dörthe, Nüsslein-Volhard, Christiane, Rauch, Gerd-Jörg
Keywords
zebrafish, deadly seven, notch1, her1, somite, segmentation, sscillator, morpholino
MeSH Terms
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Biological Clocks/physiology*
  • Homeodomain Proteins/genetics
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism*
  • Nerve Tissue Proteins/genetics
  • Receptor, Notch1
  • Receptors, Cell Surface*
  • Repressor Proteins/metabolism*
  • Signal Transduction
  • Somites/physiology*
  • Transcription Factors*
  • Zebrafish/embryology*
  • Zebrafish Proteins
PubMed
11874913
Abstract
Somite formation is thought to be regulated by an unknown oscillator mechanism that causes the cells of the presomitic mesoderm to activate and then repress the transcription of specific genes in a cyclical fashion. These oscillations create stripes/waves of gene expression that repeatedly pass through the presomitic mesoderm in a posterior-to-anterior direction. In both the mouse and the zebrafish, it has been shown that the notch pathway is required to create the stripes/waves of gene expression. However, it is not clear if the notch pathway comprises part of the oscillator mechanism or if the notch pathway simply coordinates the activity of the oscillator among neighboring cells. In the zebrafish, oscillations in the expression of a hairy-related transcription factor, her1 and the notch ligand deltaC precede somite formation. Our study focuses on how the oscillations in the expression of these two genes is affected in the mutants aei/deltaD and des/notch1, in 'morpholino knockdowns' of deltaC and her1 and in double 'mutant' combinations. This analysis indicates that these oscillations in gene expression are created by a genetic circuit comprised of the notch pathway and the notch target gene her1. We also show that a later function of the notch pathway can create a segmental pattern even in the absence of prior oscillations in her1 and deltaC expression. Supplementary data available at http://www.eb.tuebingen.mpg.de/papers/holley_dev_2002.html
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes