ZFIN ID: ZDB-PUB-020220-8
A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish
Scheer, N., Riedl, I., Warren, J.T., Kuwada, J.Y., and Campos-Ortega, J.A.
Date: 2002
Source: Mechanisms of Development   112(1-2): 9-14 (Journal)
Registered Authors: Campos-Ortega, Jose, Kuwada, John, Riedl, Iris, Scheer, Nico, Warren, James T., Jr.
Keywords: Gal4-UAS technique; kinetics; gene expression; zebrafish
MeSH Terms:
  • Animals
  • DNA-Binding Proteins
  • Fungal Proteins/chemistry*
  • Fungal Proteins/metabolism
  • Gene Expression Regulation, Developmental*
  • Hot Temperature
  • In Situ Hybridization
  • Kinetics
  • RNA/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae Proteins*
  • Temperature
  • Time Factors
  • Transcription Factors/chemistry*
  • Transcription Factors/metabolism
  • Zebrafish
PubMed: 11850174 Full text @ Mech. Dev.
Using a temperature-inducible hsp70:Gal4 activator and UAS:myc-notch1a-intra as effector, we determined quantitatively the kinetics of expression of both transgenes and analysed the effects of varying their expressivity on several phenotypic traits in the developing zebrafish. hsp70:Gal4 is transcribed within 15min after temperature-mediated induction, but Gal4 RNA decays rapidly. The Gal4 protein was found to be quite stable, as functional Gal4, which was detectable 1.5h after heat shock (HS), persisted for at least 13h. myc-notch1a-intra RNA is expressed approximately 1.5h after HS, but unlike the Gal4 RNA, it was found to be very stable; it continues to accumulate during the succeeding 17h after HS. Fully penetrant phenotypic effects are obtained after a relatively long activator induction with a 30-min HS.