PUBLICATION

A zebrafish histone variant H2A.F/Z and a transgenic H2A.F/Z:GFP fusion protein for in vivo studies of embryonic development

Authors
Pauls, S., Geldmacher-Voss, B., and Campos-Ortega, J.A.
ID
ZDB-PUB-020220-18
Date
2001
Source
Development genes and evolution   211(12): 603-610 (Journal)
Registered Authors
Campos-Ortega, Jose, Pauls, Stefan
Keywords
zebrafish; histone2A.F/Z:GFP; transgenesis; in vivo marker
MeSH Terms
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics*
  • Animals, Genetically Modified/physiology
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Histones/genetics*
  • Histones/physiology
  • Luminescent Proteins
  • Molecular Sequence Data
  • Recombinant Fusion Proteins/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
11819118 Full text @ Dev. Genes Evol.
Abstract
We have generated transgenic zebrafish lines expressing a fusion of a histone variant, H2A.F/Z, to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria. Here, we describe the molecular cloning, partial characterisation and expression of the zebrafish H2A.F/Z histone gene, as well as the construction of the transgene and its transformation into the zebrafish germ line. No abnormality can be detected in transgenic fish expressing the H2A.F/Z:GFP fusion protein. The nuclear localisation of the fusion protein correlates with the start of zygotic transcription, in that it is present in the unfertilised egg and in the cytoplasm of cells after the first cleavages, being found in some nuclei after the seventh or eighth cleavage, whereas all nuclei from the 1,000-cell stage on, i.e. after midblastula transition, contain protein. In addition to these data, we present a few examples of the many possible applications of this transgenic line for developmental studies in vivo. Electronic supplementary material to this paper can be obtained by using the Springer LINK server located at http://dx.doi.org/10.1007/s00427-001-0196-x
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes