ZFIN ID: ZDB-PUB-020218-4
High-efficiency gene transfer into adult fish: A new tool to study fin regeneration
Tawk, M., Tuil, D., Torrente, Y., Vriz, S., and Paulin, D.
Date: 2002
Source: Genesis (New York, N.Y. : 2000)   32(1): 27-31 (Journal)
Registered Authors: Vriz, Sophie
Keywords: electroporation; fin; zebrafish; Fgf receptor 1; regeneration; dominant-negative mutation; Fgf
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Electroporation/methods*
  • Gene Transfer Techniques*
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Regeneration/physiology*
  • Signal Transduction
  • Zebrafish/genetics*
  • Zebrafish/physiology
PubMed: 11835671 Full text @ Genesis
Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to " knockdown " gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporation-based approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.