PUBLICATION
High-efficiency gene transfer into adult fish: A new tool to study fin regeneration
- Authors
- Tawk, M., Tuil, D., Torrente, Y., Vriz, S., and Paulin, D.
- ID
- ZDB-PUB-020218-4
- Date
- 2002
- Source
- Genesis (New York, N.Y. : 2000) 32(1): 27-31 (Journal)
- Registered Authors
- Vriz, Sophie
- Keywords
- electroporation; fin; zebrafish; Fgf receptor 1; regeneration; dominant-negative mutation; Fgf
- MeSH Terms
-
- Electroporation/methods*
- Gene Transfer Techniques*
- Green Fluorescent Proteins
- Animals, Genetically Modified
- Luminescent Proteins
- Signal Transduction
- Animals
- Zebrafish/genetics*
- Zebrafish/physiology
- Regeneration/physiology*
- PubMed
- 11835671 Full text @ Genesis
Citation
Tawk, M., Tuil, D., Torrente, Y., Vriz, S., and Paulin, D. (2002) High-efficiency gene transfer into adult fish: A new tool to study fin regeneration. Genesis (New York, N.Y. : 2000). 32(1):27-31.
Abstract
Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to " knockdown " gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporation-based approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping