|ZFIN ID: ZDB-PUB-020218-4|
High-efficiency gene transfer into adult fish: A new tool to study fin regeneration
Tawk, M., Tuil, D., Torrente, Y., Vriz, S., and Paulin, D.
|Source:||Genesis (New York, N.Y. : 2000) 32(1): 27-31 (Journal)|
|Registered Authors:||Vriz, Sophie|
|Keywords:||electroporation; fin; zebrafish; Fgf receptor 1; regeneration; dominant-negative mutation; Fgf|
|PubMed:||11835671 Full text @ Genesis|
Tawk, M., Tuil, D., Torrente, Y., Vriz, S., and Paulin, D. (2002) High-efficiency gene transfer into adult fish: A new tool to study fin regeneration. Genesis (New York, N.Y. : 2000). 32(1):27-31.
ABSTRACTZebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to " knockdown " gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporation-based approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.
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