PUBLICATION
Genetic analysis of photoreceptor cell development in the zebrafish retina
- Authors
- Doerre, G. and Malicki, J.
- ID
- ZDB-PUB-011220-1
- Date
- 2002
- Source
- Mechanisms of Development 110(1-2): 125-138 (Journal)
- Registered Authors
- Doerre, Geoffrey, Malicki, Jarema
- Keywords
- Eye; Retina; Photoreceptor; Cell-nonautonomy; Cell¯cell interaction; Zebrafish; Mutant
- MeSH Terms
-
- Mosaicism/genetics
- Cell Communication/genetics
- Microscopy, Electron
- Photoreceptor Cells, Vertebrate/cytology*
- Photoreceptor Cells, Vertebrate/metabolism
- Phenotype
- Retinal Pigments/metabolism
- Retina/cytology
- Retina/growth & development
- Mutation
- Zebrafish/genetics*
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Cell Death/genetics
- Animals
- PubMed
- 11744374 Full text @ Mech. Dev.
Citation
Doerre, G. and Malicki, J. (2002) Genetic analysis of photoreceptor cell development in the zebrafish retina. Mechanisms of Development. 110(1-2):125-138.
Abstract
To gain insight into the genetic mechanisms of photoreceptor development, we analyzed a collection of zebrafish mutations characterized by early photoreceptor cell loss. The mutant defects impair outer segment formation and are accompanied by an abnormal distribution of visual pigments. Rods and different cone types display defects of similar severity suggesting that genetic pathways common to all photoreceptors are affected. To investigate whether these phenotypes involve cell-cell interaction defects, we analyzed genetically mosaic animals. Interaction of niezerka photoreceptors with wild-type tissues improves the survival of mutant cells and restores their elongated morphology. In contrast, cells carrying mutations in the loci brudas, elipsa, fleer, and oval retain their defective phenotypes in a wild-type environment indicating cell-autonomy. These experiments identify distinct phenotypic categories of photoreceptor mutants and indicate that zebrafish photo! receptor defects involve both cell-autonomous and cell-nonautonomous mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping