PUBLICATION
Notch signaling is required for arterial-venous differentiation during embryonic vascular development
- Authors
- Lawson, N.D., Scheer, N., Pham, V.N., Kim, C.-H., Chitnis, A.B., Campos-Ortega, J.A., and Weinstein, B.M.
- ID
- ZDB-PUB-011010-4
- Date
- 2001
- Source
- Development (Cambridge, England) 128(19): 3675-3683 (Journal)
- Registered Authors
- Campos-Ortega, Jose, Chitnis, Ajay, Kim, Cheol-Hee, Lawson, Nathan, Pham, Van, Scheer, Nico, Weinstein, Brant M.
- Keywords
- artery; notch; vein; zebrafish
- MeSH Terms
-
- Zebrafish/embryology*
- Zebrafish/genetics
- Biomarkers
- Ephrin-B2
- Zebrafish Proteins*
- Proteins/metabolism
- Female
- Cell Differentiation/physiology
- Microinjections
- Receptors, Cell Surface*
- Veins/embryology*
- Signal Transduction
- Gene Expression Regulation, Developmental
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Mutation
- Molecular Sequence Data
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism*
- Intracellular Signaling Peptides and Proteins
- Receptors, Growth Factor/metabolism
- Base Sequence
- Arteries/embryology*
- Vascular Endothelial Growth Factor Receptor-3
- Animals
- Embryonic Induction*
- Receptor Protein-Tyrosine Kinases/metabolism
- Basic Helix-Loop-Helix Transcription Factors
- PubMed
- 11585794 Full text @ Development
Citation
Lawson, N.D., Scheer, N., Pham, V.N., Kim, C.-H., Chitnis, A.B., Campos-Ortega, J.A., and Weinstein, B.M. (2001) Notch signaling is required for arterial-venous differentiation during embryonic vascular development. Development (Cambridge, England). 128(19):3675-3683.
Abstract
Recent evidence indicates that acquisition of artery or vein identity during vascular development is governed, in part, by genetic mechanisms. The artery-specific expression of a number of Notch signaling genes in mouse and zebrafish suggests that this pathway may play a role in arterial-venous cell fate determination during vascular development. We show that loss of Notch signaling in zebrafish embryos leads to molecular defects in arterial-venous differentiation, including loss of artery-specific markers and ectopic expression of venous markers within the dorsal aorta. Conversely, we find that ectopic activation of Notch signaling leads to repression of venous cell fate. Finally, embryos lacking Notch function exhibit defects in blood vessel formation similar to those associated with improper arterial-venous specification. Our results suggest that Notch signaling is required for the proper development of arterial and venous blood vessels, and that a major role of Notch signaling in blood vessels is to repress venous differentiation within developing arteries. Movies available on-line
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping