PUBLICATION

Notch signaling is required for arterial-venous differentiation during embryonic vascular development

Authors
Lawson, N.D., Scheer, N., Pham, V.N., Kim, C.-H., Chitnis, A.B., Campos-Ortega, J.A., and Weinstein, B.M.
ID
ZDB-PUB-011010-4
Date
2001
Source
Development (Cambridge, England)   128(19): 3675-3683 (Journal)
Registered Authors
Campos-Ortega, Jose, Chitnis, Ajay, Kim, Cheol-Hee, Lawson, Nathan, Pham, Van, Scheer, Nico, Weinstein, Brant M.
Keywords
artery; notch; vein; zebrafish
MeSH Terms
  • Animals
  • Arteries/embryology*
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • Biomarkers
  • Cell Differentiation/physiology
  • Embryonic Induction*
  • Ephrin-B2
  • Female
  • Gene Expression Regulation, Developmental
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Microinjections
  • Molecular Sequence Data
  • Mutation
  • Proteins/metabolism
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism*
  • Receptor Protein-Tyrosine Kinases/metabolism
  • Receptors, Cell Surface*
  • Receptors, Growth Factor/metabolism
  • Signal Transduction
  • Vascular Endothelial Growth Factor Receptor-3
  • Veins/embryology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins*
PubMed
11585794 Full text @ Development
Abstract
Recent evidence indicates that acquisition of artery or vein identity during vascular development is governed, in part, by genetic mechanisms. The artery-specific expression of a number of Notch signaling genes in mouse and zebrafish suggests that this pathway may play a role in arterial-venous cell fate determination during vascular development. We show that loss of Notch signaling in zebrafish embryos leads to molecular defects in arterial-venous differentiation, including loss of artery-specific markers and ectopic expression of venous markers within the dorsal aorta. Conversely, we find that ectopic activation of Notch signaling leads to repression of venous cell fate. Finally, embryos lacking Notch function exhibit defects in blood vessel formation similar to those associated with improper arterial-venous specification. Our results suggest that Notch signaling is required for the proper development of arterial and venous blood vessels, and that a major role of Notch signaling in blood vessels is to repress venous differentiation within developing arteries. Movies available on-line
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes