PUBLICATION
The nuclear localization signal of zebrafish terra is located within the DM domain
- Authors
- Zhang, L., Hua, Z., Ren, J., and Meng, A.
- ID
- ZDB-PUB-010912-27
- Date
- 2001
- Source
- FEBS letters 503(1): 25-29 (Journal)
- Registered Authors
- Meng, Anming, Ren, Jason (Qianshen)
- Keywords
- zebrafish; terra; DM domain; nuclear localization signal
- MeSH Terms
-
- Zinc/metabolism
- Luminescent Proteins/genetics
- Molecular Sequence Data
- Microscopy, Fluorescence
- Animals
- HeLa Cells
- DNA-Binding Proteins/chemistry
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism
- DNA Primers
- Zebrafish Proteins*
- Green Fluorescent Proteins
- Amino Acid Sequence
- Base Sequence
- Sequence Homology, Amino Acid
- Zebrafish
- Transcription Factors*
- Humans
- Nuclear Localization Signals*
- Protein Transport
- Binding Sites
- Cell Nucleus/metabolism
- PubMed
- 11513848 Full text @ FEBS Lett.
Citation
Zhang, L., Hua, Z., Ren, J., and Meng, A. (2001) The nuclear localization signal of zebrafish terra is located within the DM domain. FEBS letters. 503(1):25-29.
Abstract
Zebrafish Terra is a member of the DM domain-containing transcription factor family and is involved in somitogenesis. The other known members of this family play a role in sex differentiation across species from Caenorhabditis elegans to human. Using the green fluorescence protein-Terra fusion constructs, we have identified the nuclear localization signal (NLS) of terra by transfecting human HeLa cells. The terra NLS is located between the two intertwined zinc-binding sites of the DNA-binding domain. However, the nuclear translocation of terra is independent of the structure required for DNA binding. Mutational analysis demonstrates that basic residues K77 and R78 within the DM domain are absolutely required for the translocation of Terra into the nuclei. Sequence comparison discloses that the NLS of Terra is also present in the other known members of the DM family, indicating the conservative nature of the NLS of this family during evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping