Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer
- Gee, K.R., Weinberg, E.S., and Kozlowski, D.J.
- Bioorganic & medicinal chemistry letters 11(16): 2181-2183 (Journal)
- Registered Authors
- Kozlowski, David J., Weinberg, Eric
- MeSH Terms
- Dextrans/chemical synthesis*
- Embryo, Nonmammalian
- Fluorescent Dyes/chemical synthesis*
- Fluorescent Dyes/chemistry
- Microscopy, Fluorescence
- Rhodamines/chemical synthesis*
- 11514165 Full text @ Bioorg. Med. Chem. Lett.
Gee, K.R., Weinberg, E.S., and Kozlowski, D.J. (2001) Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer. Bioorganic & medicinal chemistry letters. 11(16):2181-2183.
An amine-reactive caged rhodamine was synthesized and conjugated to aminodextran. The resulting tracer was injected into a single cell zebrafish embryo, and a portion of the tracer was photolyzed in a single cell after development. The resulting fluorescent cell was imaged by fluorescence microscopy through a single round of cell division.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes