ZFIN ID: ZDB-PUB-010807-8
A zebrafish vasa morphant abolishes Vasa protein but does not affect the establishment of the germline
Braat, A.K., van De Water, S., Korving, J., and Zivkovic, D.
Date: 2001
Source: Genesis (New York, N.Y. : 2000)   30(3): 183-185 (Journal)
Registered Authors: Braat, Koen, Jongejan-Zivkovic, Dana
Keywords: zebrafish; vasa; morpholino; germline
MeSH Terms:
  • Animals
  • DEAD-box RNA Helicases
  • Gene Expression
  • Germ Cells/cytology
  • Germ Cells/growth & development*
  • Germ Cells/metabolism
  • Microscopy, Confocal
  • Oligonucleotides, Antisense/genetics*
  • RNA Helicases/genetics*
  • RNA Helicases/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 11477703 Full text @ Genesis
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ABSTRACT
Germline formation in the zebrafish is still poorly understood. The cloning of the zebrafish vasa cDNA (Yoon et al., 1997; Olsen et al., 1997) and analysis of localization and distribution of the vasa mRNA (Yoon et al., 1997; Olsen et al., 1997; Braat et al., 1999; Knaut et al, 2000) have demonstrated its maternal expression and its localization that is restricted to the presumptive primordial germ cells (pPGCs) and the PGCs in the embryo and larva. Unlike the vasa mRNA, the Vasa protein is uniformly distributed throughout the early embryo and can only be detected in a higher concentration from gastrulation onward in a subset of cells presumably pPGCs. Subsequently the protein becomes restricted to the PGCs (Braat et al., 2000). Although vasa mRNA and Vasa protein are localized to the PGCs, their respective significance for germline establishment or maintenance is still unclear. To investigate the function of the Vasa protein synthesis by vasa morpholino injection, which resulted in the disappearance of Vasa protein in the one-day-old zebrafish embryo as shown by immunostaining. Morphological characterization of zebrafish PGCs (Braat et al., 1999) in morpholino-injected embryos revealed cells with the typical PGC morphology, and morpholino-injected embryos raised to adulthood were capable of producing offspring.
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