PUBLICATION
Functional repression of islet-2 by disruption of complex with Ldb impairs peripheral axonal outgrowth in embryonic zebrafish
- Authors
- Segawa, H., Miyashita, T., Hirate, Y., Higashijima, S., Chino, N., Uyemura, K., Kikuchi, Y., and Okamoto, H.
- ID
- ZDB-PUB-010705-5
- Date
- 2001
- Source
- Neuron 30(2): 423-436 (Journal)
- Registered Authors
- Higashijima, Shin-ichi, Hirate, Yoshikazu, Kikuchi, Yutaka, Miyashita, Toshio, Okamoto, Hitoshi, Segawa, Hiroshi
- Keywords
- none
- MeSH Terms
-
- Homeodomain Proteins/chemistry
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Oligodeoxyribonucleotides, Antisense/pharmacology
- Axons/physiology*
- Green Fluorescent Proteins
- Genes, Reporter
- Luminescent Proteins/analysis
- Luminescent Proteins/genetics
- Mosaicism
- Neurons, Afferent/physiology*
- Transcription, Genetic
- Motor Neurons/physiology*
- RNA, Messenger/genetics
- Transcription Factors
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Binding Sites
- LIM-Homeodomain Proteins
- Embryo, Nonmammalian/physiology
- Zebrafish/embryology*
- Zebrafish/genetics*
- Protein Biosynthesis
- Animals
- PubMed
- 11395004 Full text @ Neuron
Citation
Segawa, H., Miyashita, T., Hirate, Y., Higashijima, S., Chino, N., Uyemura, K., Kikuchi, Y., and Okamoto, H. (2001) Functional repression of islet-2 by disruption of complex with Ldb impairs peripheral axonal outgrowth in embryonic zebrafish. Neuron. 30(2):423-436.
Abstract
Islet-2 is a LIM/homeodomain-type transcription factor of the Islet-1 family expressed in embryonic zebrafish. Two Islet-2 molecules bind to the LIM domain binding protein (Ldb) dimers. Overexpression of the LIM domains of Islet-2 or the LIM-interacting domain of Ldb proteins prevented binding of Islet-2 to Ldb proteins in vitro and caused similar in vivo defects in positioning, peripheral axonal outgrowth, and neurotransmitter expression by the Islet-2-positive primary sensory and motor neurons as the defects induced by injection of Islet-2-specific antisense morpholino oligonucleotide. These and other experiments, i.e., mosaic analysis, coexpression of full-length Islet-2, and overexpression of the chimeric LIM domains derived from two different Islet-1 family members, demonstrated that Islet-2 regulates neuronal differentiation by forming a complex with Ldb dimers and possibly with some other Islet-2-specific cofactors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping