PUBLICATION
Vertebrate model systems in the study of early heart development: Xenopus and zebrafish
- Authors
- Lohr, J.L. and Yost, J.
- ID
- ZDB-PUB-010522-6
- Date
- 2000
- Source
- American journal of medical genetics 97(4): 248-257 (Review)
- Registered Authors
- Yost, H. Joseph
- Keywords
- cardiac development; Xenopus; zebrafish; organogenesis; laterality; left-right development
- MeSH Terms
-
- Animals
- Drosophila Proteins*
- Drosophila melanogaster/embryology
- Drosophila melanogaster/genetics
- Embryo, Nonmammalian/physiology
- Female
- Gene Targeting/methods
- Genetic Techniques
- Genome
- Glycoproteins/genetics
- Glycoproteins/physiology
- Heart/embryology*
- Heart Defects, Congenital/embryology
- Heart Defects, Congenital/genetics
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology
- Humans
- Intracellular Signaling Peptides and Proteins
- Male
- Models, Animal*
- Models, Biological
- Morphogenesis
- Repressor Proteins/genetics
- Repressor Proteins/physiology
- Species Specificity
- Trans-Activators/genetics
- Trans-Activators/physiology
- Transcription Factors*
- Transforming Growth Factor beta/deficiency
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/physiology
- Vertebrates/embryology*
- Vertebrates/genetics
- Xenopus Proteins*
- Xenopus laevis/embryology
- Xenopus laevis/genetics
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins
- PubMed
- 11376436 Full text @ Am. J. Med. Genet.
Citation
Lohr, J.L. and Yost, J. (2000) Vertebrate model systems in the study of early heart development: Xenopus and zebrafish. American journal of medical genetics. 97(4):248-257.
Abstract
Xenopus and zebrafish serve as outstanding models in which to study vertebrate heart development. The embryos are transparent, allowing observation during organogenesis; they can be obtained in large numbers; and they are readily accessible to embryologic manipulation and microinjection of RNA, DNA, or protein. These embryos can live by diffusion for several days, allowing analysis of mutants or experimental treatments that perturb normal heart development. Xenopus embryos have been used to understand the induction of the cardiac field, the role of Nkx genes in cardiac development, and the role transforming growth factor beta molecules in the establishment and signaling of left-right axis information. Large-scale mutant screens in zebrafish and the development of transgenics in both Xenopus and zebrafish have accelerated the molecular identification of genes that regulate conserved steps in cardiovascular development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping