ZFIN ID: ZDB-PUB-010514-4
Sparking new frontiers: using in vivo electroporation for genetic manipulations
Swartz, M., Eberhart, J., Mastick, G.S., and Krull, C.E.
Date: 2001
Source: Developmental Biology 233(1): 13-21 (Review)
Registered Authors: Eberhart, Johann, Swartz, Mary
Keywords: none
MeSH Terms:
  • Animals
  • Chick Embryo
  • Electroporation/methods*
  • Embryology/methods*
  • Genetic Techniques*
  • In Vitro Techniques
  • Mice
  • Nervous System/embryology
PubMed: 11319854 Full text @ Dev. Biol.
In vivo electroporation is a fascinating new approach by which gene expression, regulation, and function can be studied in developmental systems. This technique offers new opportunities for manipulations in animal models that lack genetic approaches, including avians. Furthermore, this approach is applicable to other embryo populations including mice, ascidians, zebrafish, Xenopus, and Drosophila. In this review, we discuss technical aspects of in vivo electroporation, review recent studies where this approach has been utilized successfully, and identify future directions.