PUBLICATION
Sparking new frontiers: using in vivo electroporation for genetic manipulations
- Authors
- Swartz, M., Eberhart, J., Mastick, G.S., and Krull, C.E.
- ID
- ZDB-PUB-010514-4
- Date
- 2001
- Source
- Developmental Biology 233(1): 13-21 (Review)
- Registered Authors
- Eberhart, Johann, Swartz, Mary
- Keywords
- none
- MeSH Terms
-
- In Vitro Techniques
- Chick Embryo
- Embryology/methods*
- Mice
- Nervous System/embryology
- Electroporation/methods*
- Genetic Techniques*
- Animals
- PubMed
- 11319854 Full text @ Dev. Biol.
Citation
Swartz, M., Eberhart, J., Mastick, G.S., and Krull, C.E. (2001) Sparking new frontiers: using in vivo electroporation for genetic manipulations. Developmental Biology. 233(1):13-21.
Abstract
In vivo electroporation is a fascinating new approach by which gene expression, regulation, and function can be studied in developmental systems. This technique offers new opportunities for manipulations in animal models that lack genetic approaches, including avians. Furthermore, this approach is applicable to other embryo populations including mice, ascidians, zebrafish, Xenopus, and Drosophila. In this review, we discuss technical aspects of in vivo electroporation, review recent studies where this approach has been utilized successfully, and identify future directions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping