PUBLICATION

Regulation of steroidogenesis in transgenic mice and zebrafish

Authors
Hu, M., Chiang, E.F., Tong, S., Lai, W., Hsu, N., Wang, L.C., and Chung, B.
ID
ZDB-PUB-010219-16
Date
2001
Source
Molecular and Cellular Endocrinology   171(1-2): 9-14 (Journal)
Registered Authors
Chiang, Evelyn, Chung, Bon-chu
Keywords
embryogenesis; P450scc; side-chain cleavage enzyme; steroidogenesis; Y1 cells
MeSH Terms
  • Adrenal Glands/metabolism
  • Animals
  • Cholesterol Side-Chain Cleavage Enzyme/genetics
  • Cyclic AMP/pharmacology
  • Gene Expression Regulation*
  • Gonads/metabolism
  • Mice
  • Mice, Transgenic/genetics*
  • Models, Animal
  • Pituitary Hormones/pharmacology
  • Promoter Regions, Genetic
  • Steroids/biosynthesis*
  • Zebrafish/genetics*
PubMed
11165005 Full text @ Mol. Cell. Endocrinol.
Abstract
Steroid hormones are important physiological regulators in the body. Steroid hormones are mainly synthesized in the adrenal and gonads. Their synthesis is stimulated by pituitary hormones through cAMP as an intracellular mediator. The first and rate-limiting step for steroid biosynthesis is catalyzed by CYP11A1. Important regulatory elements for the control of the CYP11A1 gene expression have been characterized both in vitro and in vivo. The SF-1-binding sites are cis-acting elements controlling the basal and cAMP-stimulated gene expression. Our transgenic mouse studies showed that the 2.3kb promoter contains information controlling developmentally regulated gene expression. Finally, we present our results on the cloning of steroidogenic genes in zebrafish, a new model organism for genetic studies.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes