ZFIN ID: ZDB-PUB-010207-1
Isolation, characterization, and expression analysis of zebrafish large Mafs
Kajihara, M., Kawauchi, S., Kobayashi, M., Ogino, H., Takahashi, S., and Yasuda, K.
Date: 2001
Source: J. Biochem. (Tokyo)   129(1): 139-146 (Journal)
Registered Authors: Kobayashi, Makoto
Keywords: basic-leucine zipper (b-Zip); large Mafs; Maf recognition elements (MARE); somite; transcription factor
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • DNA, Complementary/analysis
  • DNA-Binding Proteins*
  • Gene Expression/physiology
  • Lens, Crystalline/physiology
  • Maf Transcription Factors, Large
  • Molecular Sequence Data
  • MyoD Protein/biosynthesis
  • Oncogene Proteins/classification
  • Oncogene Proteins/genetics
  • Oncogene Proteins/isolation & purification*
  • Phylogeny
  • RNA, Messenger/biosynthesis
  • Sequence Homology, Amino Acid
  • Somites/metabolism
  • Trans-Activators/classification
  • Trans-Activators/genetics
  • Trans-Activators/isolation & purification*
  • Transcriptional Activation
  • Zebrafish/physiology*
  • Zebrafish Proteins*
PubMed: 11134968 Full text @ J. Biochem. (Tokyo)
Large Maf proteins, which are members of the basic leucine zipper (b-Zip) superfamily, are involved in the determination and control of cellular differentiation. The expression patterns of various vertebrate large Maf mRNAs were described previously. Here, we report the cloning of a novel zebrafish large Maf cDNA, SMaf1 (Somite Maf1), and other zebrafish large Mafs, the N-terminus domains of which possess transactivational activity. We also analyzed the expression patterns of SMaf1 and SMaf2 (Somite Maf2)/Krml2 as well as MafB/Val and c-Maf during zebrafish embryogenesis. In particular, the robust expression of the novel SMaf1 mRNA, which overlapped that of MyoD, in somitic cells during somitogenesis was noteworthy. In addition, the expression patterns of SMaf2 and MafB in the blood-forming regions, and those of c-Maf and MafB in the lens cells showed spatial redundancy, although the temporal appearance of these genes at these sites differed. These data indicate that SMafs may play important roles in somitogenesis, and that Maf proteins may have overlapping and yet specific functions as to the determination and differentiation of cell lineages.