PUBLICATION
Sex- and tissue-specific expression of aspartic proteinases in Danio rerio (zebrafish)
- Authors
- Riggio, M., Scudiero, R., Filosa, S., and Parisi, E.
- ID
- ZDB-PUB-010130-4
- Date
- 2000
- Source
- Gene 260(1-2): 67-75 (Journal)
- Registered Authors
- Parisi, Elio
- Keywords
- cathepsin D; evolution; gene expression; nothepsin
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Aspartic Acid Endopeptidases/genetics*
- Base Sequence
- Blotting, Northern
- Cathepsin D/genetics
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Female
- Gene Expression Regulation, Enzymologic
- Male
- Molecular Sequence Data
- Phylogeny
- RNA/genetics
- RNA/metabolism
- Sequence Analysis, DNA
- Sex Factors
- Tissue Distribution
- Zebrafish/genetics*
- PubMed
- 11137292 Full text @ Gene
Citation
Riggio, M., Scudiero, R., Filosa, S., and Parisi, E. (2000) Sex- and tissue-specific expression of aspartic proteinases in Danio rerio (zebrafish). Gene. 260(1-2):67-75.
Abstract
Full-length zebrafish cDNAs encoding two aspartic proteinases were cloned and sequenced. One of the two cDNAs was a 1708bp product with an open reading frame of 398 amino acid residues corresponding to a cathepsin D. The other was a 1383bp product encoding a polypeptide chain of 416 amino acids homologous to nothepsin, an aspartic proteinase first identified by us in the liver of Antarctic Notothenioidei. Gene expression assessed by RT-PCR and northern blot hybridization of RNA from different tissues showed that the expression was tissue- and sex-specific. Whereas the cathepsin D gene was expressed in all the tissues examined independently of the sex, the nothepsin gene was expressed exclusively in female livers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping