|ZFIN ID: ZDB-PUB-001221-2|
Overlapping expression of zebrafish T-brain-1 and eomesodermin during forebrain development
Mione, M., Shanmugalingam, S., Kimelman, D., and Griffin, K.
|Source:||Mechanisms of Development 100(1): 93-97 (Journal)|
|Registered Authors:||Griffin, Kevin, Kimelman, David, Mione, Marina, Shanmugalingam, Shantha|
|Keywords:||T-box genes; CNS; telencephalon; zebrafish|
|PubMed:||11118891 Full text @ Mech. Dev.|
Mione, M., Shanmugalingam, S., Kimelman, D., and Griffin, K. (2001) Overlapping expression of zebrafish T-brain-1 and eomesodermin during forebrain development. Mechanisms of Development. 100(1):93-97.
ABSTRACTT-box transcription factors are important determinants of embryonic cell fate and behaviour. Two T-box genes are expressed in the developing telencephalon of several vertebrate species, including amphibia, birds and mammals. Here we report the cloning of zebrafish T-brain-1 (tbr1) and eomesodermin (eom). As a prelude to genetic studies of neuro-ectodermal fate determination we studied their expression pattern during embryogenesis and early larval development. Eom is expressed in the presumptive telencephalon from around the 4-5 somite stage in bilaterally symmetric groups of cells; the number of positive cells increases dramatically with time and encompasses the entire dorsal telencephalon by the 22 somite stage. Tbr1 is expressed from the 18 somite stage in a subset of eom-expressing cells. By 24 hpf eom and tbr1 are expressed in largely overlapping domains in the dorsal telencephalon, tbr1 is expressed in postmitotic cells whereas eomes is also expressed in proliferative ventricular zone cells. Both genes are also found in a small domain of the diencephalon bordering the telencephalon. A detailed analysis of the expression of tbr1 and eom in the brain of 4 day old larvae shows that the two T-box genes are differentially expressed in various cell populations of the developing brain.