PUBLICATION
Midline signals regulate retinal neurogenesis in zebrafish
- Authors
- Masai, I., Stemple, D.L., Okamoto, H., and Wilson, S.W.
- ID
- ZDB-PUB-000922-3
- Date
- 2000
- Source
- Neuron 27(2): 251-263 (Journal)
- Registered Authors
- Masai, Ichiro, Okamoto, Hitoshi, Stemple, Derek L., Wilson, Steve
- Keywords
- none
- MeSH Terms
-
- Growth Substances*
- Neurons/cytology*
- Neurons/metabolism
- Zebrafish/embryology*
- PAX2 Transcription Factor
- Embryonic Induction/genetics
- Visual Pathways/cytology
- Visual Pathways/embryology
- Visual Pathways/metabolism
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/genetics
- Organ Specificity/genetics
- Zebrafish Proteins*
- Cell Differentiation/genetics
- Retina/cytology
- Retina/embryology*
- Retina/metabolism
- DNA-Binding Proteins/biosynthesis*
- DNA-Binding Proteins/genetics
- Nodal Protein
- Animals
- Signal Transduction/genetics
- Signal Transduction/physiology*
- In Vitro Techniques
- Transcription Factors/biosynthesis
- Mutation
- Transforming Growth Factor beta/metabolism
- Transforming Growth Factor beta/pharmacology
- PubMed
- 10985346 Full text @ Neuron
Citation
Masai, I., Stemple, D.L., Okamoto, H., and Wilson, S.W. (2000) Midline signals regulate retinal neurogenesis in zebrafish. Neuron. 27(2):251-263.
Abstract
In zebrafish, neuronal differentiation progresses across the retina in a pattern that is reminiscent of the neurogenic wave that sweeps across the developing eye in Drosophila. We show that expression of a zebrafish homolog of Drosophila atonal, ath5, sweeps across the eye predicting the wave of neuronal differentiation. By analyzing the regulation of ath5 expression, we have elucidated the mechanisms that regulate initiation and spread of neurogenesis in the retina. ath5 expression is lost in Nodal pathway mutant embryos lacking axial tissues that include the prechordal plate. A likely role for axial tissue is to induce optic stalk cells that subsequently regulate ath5 expression. Our results suggest that a series of inductive events, initiated from the prechordal plate and progressing from the optic stalks, regulates the spread of neuronal differentiation across the zebrafish retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping