PUBLICATION
Characterization of the MTF-1 transcription factor from zebrafish and trout cells
- Authors
- Dalton, T.P., Solis, W.A., Nebert, D.W., and Carvan, M.J., III
- ID
- ZDB-PUB-000824-2
- Date
- 2000
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 126(3): 325-335 (Journal)
- Registered Authors
- Carvan III, Michael J., Nebert, Daniel W., Solis, Willy A.
- Keywords
- metal response element-binding; transcription factor-1; metallothionein; zebrafish; trout
- MeSH Terms
-
- Animals
- DNA-Binding Proteins
- Mice
- Transcription Factors/analysis
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Trout/genetics
- Trout/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 11007174 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Dalton, T.P., Solis, W.A., Nebert, D.W., and Carvan, M.J., III (2000) Characterization of the MTF-1 transcription factor from zebrafish and trout cells. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 126(3):325-335.
Abstract
The metal response element (MRE)-binding transcription factor-1, MTF-1, is a zinc-responsive protein that controls transcription of metallothionein (MT) genes in many cell types. In addition, MTF-1 is also hypothesized to regulate transcription of a battery of genes involved in the defense against oxidative stress. Manipulating the Zn concentration in the low muM range reversibly modulates the DNA-binding activity of the mammalian MTF-1; this effect is inhibited at low temperature. This report examines the presence and binding properties of MTF-1 in cell lines derived from warm- and cold-water fishes (zebrafish and trout, respectively). We found that both species of fish express MRE-specific binding activities that are immunologically similar to mouse MTF-1. MTF-1-binding from the cells of both species of fish was activated when cells were treated with Zn but not with Cd. Zebrafish cells contained a single isoform of MTF-1 with binding properties similar to mammalian MTF-1. Trout cells, on the other hand, contained two isoforms of MTF-1: MTF-1H and MTF-1L. Zn reversibly modulated MTF-1H binding in a temperature-dependent manner. Similarly, Zn reversibly modulated MTF-1L binding, but, in contrast, such modulation occurred readily at 4 degrees C. This data demonstrate the conservation of binding specificity, binding properties, and regulation of MTF-1 in fishes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping