PUBLICATION
Characterization of ZFOR1, a putative delta-opioid receptor from the teleost zebrafish (Danio rerio)
- Authors
- Rodriguez, R.E., Barrallo, A., Garcia Malvar, F., McFadyen, I.J., González Sarmiento, R., and Traynor, J.R.
- ID
- ZDB-PUB-000810-5
- Date
- 2000
- Source
- Neuroscience letters 288(3): 207-210 (Journal)
- Registered Authors
- Barrallo Gimeno, Alejandro, González Sarmiento, Rogelio, Rodriguez, Raquel E.
- Keywords
- delta-opioid receptor; zebrafish; opioid binding; [S-35]GTPgamma S assays; HEK cells
- MeSH Terms
-
- Animals
- Binding Sites/drug effects
- Diprenorphine/pharmacology
- Humans
- Narcotic Antagonists/pharmacology
- Receptors, Opioid, delta/biosynthesis
- Receptors, Opioid, delta/chemistry*
- Receptors, Opioid, delta/metabolism
- Sequence Homology, Amino Acid
- Tritium
- Zebrafish/metabolism*
- Zebrafish/physiology*
- PubMed
- 10889344 Full text @ Neurosci. Lett.
Citation
Rodriguez, R.E., Barrallo, A., Garcia Malvar, F., McFadyen, I.J., González Sarmiento, R., and Traynor, J.R. (2000) Characterization of ZFOR1, a putative delta-opioid receptor from the teleost zebrafish (Danio rerio). Neuroscience letters. 288(3):207-210.
Abstract
ZFOR1 is a putative opioid receptor from zebrafish brain which has 66% homology with the mammalian delta-opioid receptor. When expressed in HEK293 cells ZFOR1 bound the non-selective opioid antagonist [(3)H]diprenorphine with high affinity. However, the binding of this ligand was not readily displaced by opioids selective for μ, delta or kappa opioid receptors (affinities>1000 nM). Rather non-selective ligands showed good affinity, as did the non-peptide delta-ligand BW373U86 (Ki 69 nM), the delta-antagonist naltrindole (Ki 28 nM) and the peptide beta-endorphin (Ki 37 nM). Agonist binding to the receptor encoded by ZFOR1 receptor stimulated the binding of [(35)S]GTPgammaS confirming coupling to G proteins. Study of the receptor should contribute to understanding of the evolution of the opioid system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping