PUBLICATION
Identification and linkage of the proteasome activator complex PA28 subunit genes in zebrafish
- Authors
- Murray, B.W., Sültmann, H., and Klein, J.
- ID
- ZDB-PUB-000623-2
- Date
- 2000
- Source
- Scandinavian journal of immunology 51(6): 571-576 (Journal)
- Registered Authors
- Klein, Jan, Sültmann, Holger
- Keywords
- none
- MeSH Terms
-
- Zebrafish Proteins*
- Molecular Sequence Data
- Zebrafish
- Proteins/genetics*
- Proteins/isolation & purification*
- Proteins/metabolism
- Sequence Alignment
- Amino Acid Sequence
- Cysteine Endopeptidases/genetics*
- Cysteine Endopeptidases/isolation & purification
- Multigene Family
- Cloning, Molecular
- Genetic Linkage/genetics*
- Multienzyme Complexes/genetics*
- Multienzyme Complexes/isolation & purification
- Multienzyme Complexes/metabolism
- Animals
- PubMed
- 10849367 Full text @ Scand. J. Immunol.
Citation
Murray, B.W., Sültmann, H., and Klein, J. (2000) Identification and linkage of the proteasome activator complex PA28 subunit genes in zebrafish. Scandinavian journal of immunology. 51(6):571-576.
Abstract
PA28 is an activator of the latent 20S proteasome, a large multisubunit complex involved in intracellular proteolysis. Two forms of hexameric PA28 have been identified, PA28-(alphabeta)3 and PA28-(gamma)6, of which the former is of immunological importance. Both the PA28-alpha and PA28-beta subunits are inducible by interferon-gamma (IFN-gamma) and the PA28-(alphabeta)3 complex enhances the ability of the 20S proteasome to produce peptides suited for binding to major histocompatibility complex (Mhc) class I molecules. To identify the homologues of the PA28 subunits in zebrafish we screened a cDNA library and obtained full-length cDNA sequences of the genes PSME1, PSME2 and PSME3 coding for the PA28-alpha, PA28-beta and PA28-gamma subunits, respectively. Phylogenetic analysis indicates the existence of the ancestors of all three genes prior to the divergence of tetrapods and bony fishes. The IFN-gamma-inducible subunits, PA28-alpha and PA28-beta, evolve faster than the presumably older PA28-gamma subunit. Using zebrafish radiation hybrid panels, the genes PSME2 and PSME3 were mapped to linkage group 12 and shown to be separated by a distance of less than 2.4 cM. This observation suggests that an intrachromosomal duplication event created the precursor of the IFN-gamma-inducible genes from a PA28-gamma-like ancestor prior to their recruitment into the Mhc class I peptide presentation pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping