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ZFIN ID: ZDB-PUB-000622-5
cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebrafish (Danio rerio)
Denovan-Wright, E.M., Pierce, M., Sharma, M.K., and Wright, J.M.
Date: 2000
Source: BBA Gene Structure and Expression   1492(1): 227-232 (Journal)
Registered Authors: Sharma, Mukesh, Wright, Jonathan M.
Keywords: liver basic fatty acid binding protein; zebrafish; cDNA cloning; in situ hybridization; Northern analysis
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins/biosynthesis
  • Carrier Proteins/genetics*
  • Cloning, Molecular
  • DNA, Complementary/analysis
  • Fatty Acid-Binding Proteins
  • Gene Expression
  • Humans
  • In Situ Hybridization
  • Liver/metabolism*
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Proteins*
  • Zebrafish
  • Zebrafish Proteins*
PubMed: 11004494 Full text @ BBA Gene Structure and Expression
FIGURES
ABSTRACT
We have determined the nucleotide sequence for a cDNA clone derived from zebrafish (Danio rerio) that codes for a fatty acid binding protein (FABP). Amino acid sequence similarity of the zebrafish FABP was highest to FABPs isolated from the livers of catfish, chicken, salamander and iguana. The open-reading frame of the zebrafish FABP cDNA codes for a protein of 14.0 kDa with a calculated isoelectric point of 8.8. The zebrafish liver-type FABP (L-FABP) cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases HaeIII or EcoRI suggesting that a single copy of the L-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish L-FABP cDNA hybridized to a transcript of 700 nucleotides in total zebrafish RNA. In situ hybridization and emulsion autoradiography revealed that the L-FABP was expressed exclusively in the liver of the adult zebrafish. Based on amino acid sequence similarity, the isoelectric point and its tissue-specific pattern of expression, we conclude that this zebrafish FABP belongs to the basic liver-type FABPs only found, thus far, in non-mammalian vertebrates.
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