PUBLICATION
Deafferentation-induced changes in the olfactory bulb of adult zebrafish
- Authors
- Byrd, C.A.
- ID
- ZDB-PUB-000621-1
- Date
- 2000
- Source
- Brain research 866(1-2): 92-100 (Journal)
- Registered Authors
- Byrd-Jacobs, Christine
- Keywords
- primary afferents; olfactory nerve; denervation; ablation; tyrosine hydroxylase
- MeSH Terms
-
- Animals
- Axons/metabolism
- Axons/ultrastructure
- Denervation/adverse effects
- Models, Biological
- Nerve Degeneration/pathology
- Nerve Degeneration/physiopathology
- Olfactory Bulb/cytology
- Olfactory Bulb/physiology*
- Olfactory Mucosa/cytology
- Olfactory Mucosa/physiology*
- Organ Size/physiology
- Tyrosine 3-Monooxygenase/metabolism
- Zebrafish/anatomy & histology
- Zebrafish/physiology*
- PubMed
- 10825484 Full text @ Brain Res.
Citation
Byrd, C.A. (2000) Deafferentation-induced changes in the olfactory bulb of adult zebrafish. Brain research. 866(1-2):92-100.
Abstract
The influence of the olfactory organ on maintenance of olfactory bulb structure was examined in zebrafish, using peripheral deafferentation. This fish provides a model in which the olfactory organ is easily accessible for removal, the animals easily survive the surgery, and the olfactory bulbs are small enough to allow rigorous analysis of the resulting effects. Unilateral olfactory organ ablations were performed on anesthetized adult zebrafish using a small-vessel cautery iron. Fish were allowed to survive for 1, 3, or 6 weeks following the procedure. Analysis of deafferented animals revealed that most, if not all, of the olfactory organ was missing on the ablated side, and the structure did not regenerate. The morphology of the olfactory bulb was affected notably by the removal of its primary afferent innervation. The olfactory nerve layer was diminished at 1 week and absent by 3 weeks post-deafferentation. At all of the survival times the deafferented bulb appeared significantly smaller at the gross level, and there was a statistically significant effect on bulb size and cell number after 6 weeks. Tyrosine hydroxylase expression, as revealed by immunohistochemistry, was decreased noticeably on the ablated side. In conclusion, the olfactory organ is important in the preservation of normal olfactory bulb anatomy and neurochemistry in adult zebrafish. Thus, the influence of the periphery does not end with the formation of the mature olfactory bulb.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping