PUBLICATION
Cloning and characterization of cDNA encoding zebrafish Danio rerio NM23-B gene
- Authors
- Lee, J.S. and Lee, S.H.
- ID
- ZDB-PUB-000405-2
- Date
- 2000
- Source
- Gene 245(1): 75-79 (Journal)
- Registered Authors
- Lee, Jae-Seong
- Keywords
- cloning; Danio rerio; NM23-B; zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Cross-Linking Reagents
- DNA, Complementary/chemistry
- DNA, Complementary/genetics*
- DNA, Single-Stranded/genetics
- DNA, Single-Stranded/metabolism
- Female
- Gene Expression
- Molecular Sequence Data
- Monomeric GTP-Binding Proteins/genetics*
- Monomeric GTP-Binding Proteins/metabolism
- NM23 Nucleoside Diphosphate Kinases
- Nucleoside-Diphosphate Kinase*
- Oligonucleotides/genetics
- Oligonucleotides/metabolism
- Protein Binding
- RNA/genetics
- RNA/metabolism
- Repetitive Sequences, Nucleic Acid
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Telomere/genetics
- Telomere/metabolism
- Tissue Distribution
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Ultraviolet Rays
- Zebrafish/genetics*
- PubMed
- 10713447 Full text @ Gene
Citation
Lee, J.S. and Lee, S.H. (2000) Cloning and characterization of cDNA encoding zebrafish Danio rerio NM23-B gene. Gene. 245(1):75-79.
Abstract
A full-length zebrafish NM23-B cDNA was cloned and sequenced. The zebrafish NM23-B cDNA consists of 624bp with an open reading frame of 153 amino acids. NM23-B mRNA of approximately 0.7kb is present in adult zebrafish tissues. Zebrafish NM23-B his-tagged protein (17kDa) was produced in E. coli and characterized by binding and UV-cross-linking to a single-stranded telomeric repeat (TTAGGG)(6). This is the first report to show that fish have a NM23-H2 homologue that is similar to that in humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping