Expression of sox11 gene duplicates in zebrafish suggests the reciprocal loss of ancestral gene expression patterns in development

De Martino, S., Yan, Y.-L., Jowett, T., Postlethwait, J.H., Varga, Z.M., Ashworth, A., and Austin, C.A.
Developmental Dynamics : an official publication of the American Association of Anatomists   217(3): 279-292 (Journal)
Registered Authors
Austin, Caroline Ann, Jowett, Trevor, Postlethwait, John H., Varga, Zoltán M., Yan, Yi-Lin
Danio rerio; sox 11; gene evolution
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Central Nervous System/embryology
  • Central Nervous System/metabolism
  • Chickens
  • Chromosome Mapping
  • DNA, Complementary
  • Gene Duplication*
  • Gene Expression
  • High Mobility Group Proteins/classification
  • High Mobility Group Proteins/genetics*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • SOX Transcription Factors
  • SOXC Transcription Factors
  • Sequence Homology, Amino Acid
  • Xenopus
  • Xenopus Proteins*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins*
10741422 Full text @ Dev. Dyn.
To investigate the role of sox genes in vertebrate development, we have isolated sox11 from zebrafish (Danio rerio). Two distinct classes of sox11-related cDNAs were identified, sox11a and sox11b. The predicted protein sequences shared 75% identity. In a gene phylogeny, both sox11a and sox11b cluster with human, mouse, chick, and Xenopus Sox11, indicating that zebrafish, like Xenopus, has two orthologues of tetrapod Sox11. The work reported here investigates the evolutionary origin of these two gene duplicates and the consequences of their duplication for development. The sox11a and sox11b genes map to linkage groups 17 and 20, respectively, together with other loci whose orthologues are syntenic with human SOX11, suggesting that during the fish lineage, a large chromosome region sharing conserved syntenies with mammals has become duplicated. Studies in mouse and chick have shown that Sox11 is expressed in the central nervous system during development. Expression patterns of zebrafish sox11a and sox11b confirm that they are expressed in the developing nervous system, including the forebrain, midbrain, hindbrain, eyes, and ears from an early stage. Other sites of expression include the fin buds and somites. The two sox genes, sox11a and sox11b, are expressed in both overlapping and distinct sites. Their expression patterns suggest that sox11a and sox11b may share the developmental domains of the single Sox11 gene present in mouse and chick. For example, zebrafish sox11a is expressed in the anterior somites, and zebrafish sox11b is expressed in the posterior somites, but the single Sox11 gene of mouse is expressed in all the somites. Thus, the zebrafish duplicate genes appear to have reciprocally lost expression domains present in the sox11 gene of the last common ancestor of tetrapods and zebrafish. This splitting of the roles of Sox11 between two paralogues suggests that regulatory elements governing the expression of the sox11 gene in the common ancestor of zebrafish and tetrapods may have been reciprocally mutated in the zebrafish gene duplicates. This is consistent with duplicate gene evolution via a duplication-degeneration-complementation process.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes