ZFIN ID: ZDB-PERS-980202-13
Rawls, John F.
Email: john.rawls@duke.edu
URL: http://rawlslab.duhs.duke.edu/
Affiliation: Rawls Lab
Address: Department of Molecular Genetics & Microbiology Duke University Medical Center 213 Research Drive 323 CARL Building, Box 3580 Durham, NC 27710 USA
Country: United States
Phone: (919) 613-7212
Fax: (919) 684-2790
Orcid ID:


BIOGRAPHY AND RESEARCH INTERESTS


PUBLICATIONS
Cronan, M.R., Matty, M.A., Rosenberg, A.F., Blanc, L., Pyle, C.J., Espenschied, S.T., Rawls, J.F., Dartois, V., Tobin, D.M. (2018) An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas. Nature Methods. 15:1098-1107
Minchin, J.E.N., Scahill, C.M., Staudt, N., Busch-Nentwich, E.M., Rawls, J.F. (2018) Deep phenotyping in zebrafish reveals genetic and diet-induced adiposity changes that may inform disease risk. Journal of Lipid Research. 59(8):1536-1545
King, J., Foster, J., Davison, J.M., Rawls, J.F., Breton, G. (2017) Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation. Zebrafish. 15(2):202-205
Phelps, D., Brinkman, N.E., Keely, S.P., Anneken, E.M., Catron, T.R., Betancourt, D., Wood, C.E., Espenschied, S.T., Rawls, J.F., Tal, T. (2017) Microbial colonization is required for normal neurobehavioral development in zebrafish. Scientific Reports. 7:11244
Lickwar, C.R., Camp, J.G., Weiser, M., Cocchiaro, J.L., Kingsley, D.M., Furey, T.S., Sheikh, S.Z., Rawls, J.F. (2017) Genomic dissection of conserved transcriptional regulation in intestinal epithelial cells. PLoS Biology. 15:e2002054
Minchin, J.E.N., Rawls, J.F. (2017) Elucidating the role of plexin D1 in body fat distribution and susceptibility to metabolic disease using a zebrafish model system. Adipocyte. 6(4):277-283
Davison, J.M., Lickwar, C.R., Song, L., Breton, G., Crawford, G.E., Rawls, J.F. (2017) Microbiota regulate intestinal epithelial gene expression by suppressing the transcription factor Hepatocyte nuclear factor 4 alpha. Genome research. 27(7):1195-1206
Minchin, J.E., Rawls, J.F. (2017) A classification system for zebrafish adipose tissues. Disease models & mechanisms. 10(6):797-809
Melancon, E., Gomez De La Torre Canny, S., Sichel, S., Kelly, M., Wiles, T.J., Rawls, J.F., Eisen, J.S., Guillemin, K. (2017) Best practices for germ-free derivation and gnotobiotic zebrafish husbandry. Methods in cell biology. 138:61-100
Minchin, J.E., Rawls, J.F. (2017) In vivo imaging and quantification of regional adiposity in zebrafish. Methods in cell biology. 138:3-27
Bae, S., Mueller, O., Wong, S., Rawls, J.F., Valdivia, R.H. (2016) Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe. Proc. Natl. Acad. Sci. USA. 113:14127-14132
Burns, A.R., Zac Stephens, W., Stagaman, K., Wong, S., Rawls, J.F., Guillemin, K., Bohannan, B.J. (2016) Contribution of neutral processes to the assembly of gut microbial communities in the zebrafish over host development. The ISME Journal. 10(3):655-64
Wong, S., Stephens, W.Z., Burns, A.R., Stagaman, K., David, L.A., Bohannan, B.J., Guillemin, K., Rawls, J.F. (2015) Ontogenetic Differences in Dietary Fat Influence Microbiota Assembly in the Zebrafish Gut. mBio. 6(5):e00687-15
Wang, L., Oehlers, S.H., Espenschied, S.T., Rawls, J.F., Tobin, D.M., Ko, D.C. (2015) CPAG: software for leveraging pleiotropy in GWAS to reveal similarity between human traits links plasma fatty acids and intestinal inflammation. Genome biology. 16:190
Minchin, J.E., Dahlman, I., Harvey, C.J., Mejhert, N., Singh, M.K., Epstein, J.A., Arner, P., Torres-Vazquez, J., Rawls, J.F. (2015) Plexin D1 determines body fat distribution by regulating the type V collagen microenvironment in visceral adipose tissue. Proc. Natl. Acad. Sci. USA. 112(14):4363-8
Marjoram, L., Alvers, A., Deerhake, M.E., Bagwell, J., Mankiewicz, J., Cocchiaro, J.L., Beerman, R.W., Willer, J., Sumigray, K.D., Katsanis, N., Tobin, D.M., Rawls, J.F., Goll, M.G., Bagnat, M. (2015) Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proc. Natl. Acad. Sci. USA. 112(9):2770-5
Kanther, M., Tomkovich, S., Xiaolun, S., Grosser, M.R., Koo, J., Flynn, E.J., Jobin, C., and Rawls, J.F. (2014) Commensal microbiota stimulate systemic neutrophil migration through induction of Serum amyloid A. Cellular Microbiology. 16(7):1053-67
Gratacap, R.L., Rawls, J.F., and Wheeler, R.T. (2013) Mucosal candidiasis elicits NF-κB activation, proinflammatory gene expression and localized neutrophilia in zebrafish. Disease models & mechanisms. 6(5):1260-70
Minchin, J.E., Williams, V.C., Hinits, Y., Low, S., Tandon, P., Fan, C.M., Rawls, J.F., and Hughes, S.M. (2013) Oesophageal and sternohyal muscle fibres are novel Pax3-dependent migratory somite derivatives essential for ingestion. Development (Cambridge, England). 140(14):2972-2984
Sadler, K.C., Rawls, J.F., and Farber, S.A. (2013) Getting the inside tract: new frontiers in zebrafish digestive system biology. Zebrafish. 10(2):129-131
McMenamin, S.K., Minchin, J.E., Gordon, T.N., Rawls, J.F., and Parichy, D.M. (2013) Dwarfism and Increased Adiposity in the gh1 Mutant Zebrafish vizzini. Endocrinology. 154(4):1476-87
Onyiah, J.C., Sheikh, S.Z., Maharshak, N., Steinbach, E.C., Russo, S.M., Kobayashi, T., Mackey, L.C., Hansen, J.J., Moeser, A.J., Rawls, J.F., Borst, L.B., Otterbein, L.E., and Plevy, S.E. (2013) Carbon Monoxide and Heme Oxygenase-1 Prevent Intestinal Inflammation in Mice by Promoting Bacterial Clearance. Gastroenterology. 144(4):789-798
Cocchiaro, J.L., and Rawls, J.F. (2013) Microgavage of zebrafish larvae. Journal of visualized experiments : JoVE. (72):e4434
Goldsmith, J.R., Cocchiaro, J.L., Rawls, J.F., and Jobin, C. (2013) Zebrafish glafenine-intestinal injury is ameliorated by mu-opioid signaling via enhancement of Atf6-dependent cellular stress responses. Disease models & mechanisms. 6(1):146-159
Semova, I., Carten, J.D., Stombaugh, J., Mackey, L.C., Knight, R., Farber, S.A., and Rawls, J.F. (2012) Microbiota regulate intestinal absorption and metabolism of Fatty acids in the zebrafish. Cell Host & Microbe. 12(3):277-288
Camp, J.G., Jazwa, A.L., Trent, C.M., and Rawls, J.F. (2012) Intronic Cis-Regulatory Modules Mediate Tissue-Specific and Microbial Control of angptl4/fiaf Transcription. PLoS Genetics. 8(3):e1002585
Roeselers, G., Mittge, E.K., Stephens, W.Z., Parichy, D.M., Cavanaugh, C.M., Guillemin, K., and Rawls, J.F. (2011) Evidence for a core gut microbiota in the zebrafish. The ISME Journal. 5(10):1595-608
Kanther, M., Sun, X., Mühlbauer, M., Mackey, L.C., Flynn, E.J., Bagnat, M., Jobin, C., and Rawls, J.F. (2011) Microbial colonization induces dynamic temporal and spatial patterns of NF-kappaB activation in the zebrafish digestive tract. Gastroenterology. 141(1):197-207
Milligan-Myhre, K., Charette, J.R., Phennicie, R.T., Stephens, W.Z., Rawls, J.F., Guillemin, K., and Kim, C.H. (2011) Study of host-microbe interactions in zebrafish. Methods in cell biology. 105:87-116
Minchin, J.E., and Rawls, J.F. (2011) In vivo Analysis of White Adipose Tissue in Zebrafish. Methods in cell biology. 105:63-86
Kanther, M., and Rawls, J.F. (2010) Host-microbe interactions in the developing zebrafish. Current opinion in immunology. 22(1):10-19
Volkman, H.E., Pozos, T.C., Zheng, J., Davis, J.M., Rawls, J.F., and Ramakrishnan, L. (2010) Tuberculous Granuloma Induction via Interaction of a Bacterial Secreted Protein with Host Epithelium. Science (New York, N.Y.). 327(5964):466-469
Flynn, E.J. 3rd, Trent, C.M., and Rawls, J.F. (2009) Ontogeny and nutritional control of adipogenesis in zebrafish (Danio rerio). Journal of Lipid Research. 50(8):1641-1652
Camp, J.G., Kanther, M., Semova, I., and Rawls, J.F. (2009) Patterns and scales in gastrointestinal microbial ecology. Gastroenterology. 136(6):1989-2002
Pham, L.N., Kanther, M., Semova, I., and Rawls, J.F. (2008) Methods for generating and colonizing gnotobiotic zebrafish. Nature Protocols. 3(12):1862-1875
Rawls, J.F., Mahowald, M.A., Goodman, A.L., Trent, C.M., and Gordon, J.I. (2007) In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut. Proc. Natl. Acad. Sci. USA. 104(18):7622-7627
Rawls, J.F., Mahowald, M.A., Ley, R.E., and Gordon, J.I. (2006) Reciprocal Gut Microbiota Transplants from Zebrafish and Mice to Germ-free Recipients Reveal Host Habitat Selection. Cell. 127(20:423-433
Rawls, J.F., Samuel, B.S., and Gordon, J.I. (2004) Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota. Proc. Natl. Acad. Sci. USA. 101(13):4596-4601
Rawls, J.F. and Johnson, S.L. (2003) Temporal and molecular separation of the kit receptor tyrosine kinase's roles in zebrafish melanocyte migration and survival. Developmental Biology. 262(1):152-161
Rawls, J.F., Frieda, M.R., McAdow, A.R., Gross, J.P., Clayton, C.M., Heyen, C.K., and Johnson, S.L. (2003) Coupled mutagenesis screens and genetic mapping in zebrafish. Genetics. 163(3):997-1009
Rawls, J.F., Mellgren, E.M., and Johnson, S.L. (2001) How the zebrafish gets its stripes. Developmental Biology. 240(2):301-314
Rawls, J.F. and Johnson, S.L. (2001) Requirements for the kit receptor tyrosine kinase during regeneration of zebrafish fin melanocytes. Development (Cambridge, England). 128(11):1943-1949
Rawls, J.F. (2001) Genetic dissection of kit-dependent melanocyte development in zebrafish, Danio rerio. Ph.D. Thesis.
Parichy, D.M., Mellgren, E.M., Rawls, J.F., Lopes, S.S., Kelsh, R.N., and Johnson, S.L. (2000) Mutational analysis of endothelin receptor b1 (rose) during neural crest and pigment pattern development in the zebrafish Danio rerio. Developmental Biology. 227(2):294-306
Rawls, J.F. and Johnson, S.L. (2000) Zebrafish kit mutation reveals primary and secondary regulation of melanocyte development during fin stripe regeneration. Development (Cambridge, England). 127(17):3715-3724
Parichy, D.M., Rawls, J.F., Pratt, S.J., Whitfield, T.T., and Johnson, S.L. (1999) Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development. Development (Cambridge, England). 126(15):3425-3436

NON-ZEBRAFISH PUBLICATIONS
Bear, J.E., Rawls, J.F., and Saxe, C.L. III. (1998) SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development. J Cell Biol.142 (5): 1325-1335.


Shen XJ, Rawls JF, Randall T, Burcal L, Mpande CN, Jenkins N, Jovov B, Abdo Z, Sandler RS, Keku TO.
Molecular characterization of mucosal adherent bacteria and associations with colorectal adenomas.
Gut Microbes. 2010 May-Jun;1(3):138-47. Epub 2010 May 13.


Sanapareddy N, Legge RM, Jovov B, McCoy A, Burcal L, Araujo-Perez F, Randall TA, Galanko J, Benson A, Sandler RS, Rawls JF, Abdo Z, Fodor AA, Keku TO.
Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans.
ISME J. 2012 May 24. doi: 10.1038/ismej.2012.43. [Epub ahead of print]