ZFIN ID: ZDB-PERS-970214-2
Buhler, Donald R.
Email: donald.buhler@orst.edu
Address: Department of Environmental and Molecular Toxicology ALS 1007 Oregon State University Corvallis, OR 97331-7301 USA
Phone: (541) 737-1784
Fax: (541) 737-0497
Orcid ID:


Spitsbergen, J.M., Buhler, D.R., and Peterson, T.S. (2012) Neoplasia and neoplasm-associated lesions in laboratory colonies of zebrafish emphasizing key influences of diet and aquaculture system design. ILAR journal. 53(2):114-125
Timme-Laragy, A.R., Noyes, P.D., Buhler, D.R., and Di Giulio, R.T. (2008) CYP1B1 knockdown does not alter synergistic developmental toxicity of polycyclic aromatic hydrocarbons in zebrafish (Danio rerio). Marine Environmental Research. 66(1):85-87
Miranda, C.L., Chung, W.G., Wang-Buhler, J.L., Musafia-Jeknic, T., Baird, W.M., and Buhler, D.R. (2006) Comparative in vitro metabolism of benzo[a]pyrene by recombinant zebrafish CYP1A and liver microsomes from beta-naphthoflavone-treated rainbow trout. Aquatic toxicology (Amsterdam, Netherlands). 80(2):101-108
Corley-Smith, G.E., Su, H.T., Wang-Buhler, J.L., Tseng, H.P., Hu, C.H., Hoang, T., Chung, W.G., and Buhler, D.R. (2006) CYP3C1, the first member of a new cytochrome P450 subfamily found in zebrafish (Danio rerio). Biochemical and Biophysical Research Communications. 340(4):1039-46
Lam, S.H., Wu, Y.L., Vega, V.B., Miller, L.D., Spitsbergen, J., Tong, Y., Zhan, H., Govindarajan, K.R., Lee, S., Mathavan, S., Krishna Murthy, K.R., Buhler, D.R., Liu, E.T., and Gong, Z. (2006) Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression. Nat. Biotechnol.. 24(1):73-75
Tseng, H.P., Hseu, T.H., Buhler, D.R., Wang, W.D., and Hu, C.H. (2005) Constitutive and xenobiotics-induced expression of a novel CYP3A gene from zebrafish larva. Toxicol. Appl. Pharmacol.. 205(3):247-258
Wang-Buhler, J.L., Lee, S.J., Chung, W.G., Stevens, J.F., Tseng, H.P., Hseu, T.H., Hu, C.H., Westerfield, M., Yang, Y.H., Miranda, C.L., and Buhler, D.R. (2005) CYP2K6 from zebrafish (Danio rerio): Cloning, mapping, developmental/tissue expression, and aflatoxin B1 activation by baculovirus expressed enzyme. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 140(2):207-219
Chung, W.G., Sen, A., Wang-Buhler, J.L., Yang, Y.H., Lopez, N., Merrill, G.F., Miranda, C.L., Hu, C.H., and Buhler, D.R. (2004) cDNA-directed expression of a functional zebrafish CYP1A in yeast. Aquatic toxicology (Amsterdam, Netherlands). 70(2):111-121
Wang, W.D., Wang, Y., Wen, H.J., Buhler, D.R., and Hu, C.H. (2004) Phenylthiourea as a weak activator of aryl hydrocarbon receptor inhibiting 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced CYP1A1 transcription in zebrafish embryo. Biochemical pharmacology. 68(1):63-71
Troxel, C.M., D.R. Buhler, J.D. Hendricks, and G.S. Bailey (1997) CYP1A Induction by β-Naphthoflavone, Aroclor 1254, and 2,3,7,8-Tetrachlorodibenzo-p-dioxin and Its Influence on Aflatoxin B1Metabolism and DNA Adduction in Zebrafish (Danio rerio). Toxicol. Appl. Pharmacol.. 146(1):69-78
Collodi, P., Miranda, C.L., Zhao, X., Buhler, D.R., and Barnes, D.W. (1994) Induction of zebrafish (Brachydanio rerio) P450 in vivo and in cell culture. Xenobiotica; the fate of foreign compounds in biological systems. 24:487-493
Buchmann, A., Wannemacher, R., Kulzer, E., Buhler, D.R., and Bock, K.W. (1993) Immunohistochemical localization of the cytochrome P450 isozymes LMC2 and LM4B (P4501A1) in 2,3,7,8-tetrachlorodibenzo-p-dioxin-treated zebrafish (Brachydanio rerio). Toxicol. Appl. Pharmacol.. 123:160-169
Miranda, C.L., Collodi, P., Zhao, X., Barnes, D.W., and Buhler, D.R. (1993) Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio). Archives of biochemistry and biophysics. 305:320-327
Collodi, P., Kamei, Y., Ernst, T., Miranda, C., Buhler, D.R., and Barnes, D.W. (1992) Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell biology and toxicology. 8(1):43-61

BUHLER, D.R., YANG, Y.-H., DREHER, T.W., MIRANDA, C.L. and WANG, J.-L. (1994). Cloning, sequencing, and expression of the major rainbow trout constitutive cytochrome P450 (CYP2K1). Identification of a new cytochrome P450 gene subfamily. Arch. Biochem. Biophys.312, 45-51.

BUHLER, D.R., WANG, J.-L., ZHAO, X., REED, R.L., HENDERSON, M.L. and MIRANDA, C.L. (1995). The effects of a luteinizing hormone-releasing hormone analogue on cytochrome P450 in rainbow trout. Mar. Environ. Res. 39, 93-96.

TAKAHASHI, N., MIRANDA, C.L., HENDERSON, M.C., BUHLER, D.R., WILLIAMS, D.E. and BAILEY, G.S. (1995). Inhibition of in vitro aflatoxin B1-DNA binding in rainbow trout by CYP1A1 inhibitors: -naphthoflavone, -naphthoflavone and trout CYP1A1 peptide antibody. Comp. Biochem. Physiol. Pharmacol. Toxicol. 110, 273-280.

CELANDER, M., BUHLER, D.R., FORLIN, L., GOKSOYR, A., MIRANDA, C.L., WOODIN, B.R. and STEGEMAN, J.J. (1996). Immunochemical relationships of cytochrome P4503A-like proteins in teleost fish. Fish Physiol. Biochem. 15, 323-332.

MIRANDA, C.L., HENDERSON, M.C., WILLIAMS, D.E. and BUHLER, D.R. (1997). In vitrometabolism of 7,12-dimethylbenz[a]anthracene by rainbow trout liver microsomes and trout P450 isoforms. Toxicol. Appl. Pharmacol. 142, 123-132.

WANG-BUHLER, J.-L., ZHAO, X., YANG, Y-H., MIRANDA, C.L. and BUHLER, D.R. (1997) Expression of a constitutive cytochrome P450 (CYP2K1) in livers of rainbow trout (Oncorhynchus mykiss) embryo and sac-fry. Aquatic Toxicol. 37, 237-251.

GOONERATNE, R., MIRANDA, C.L., HENDERSON, M.C. and BUHLER, D.R. (1997). Purification of two CYP1A proteins from BNF-treated rainbow trout. Xenobiotica 27, 175-187.

BUHLER, D.R., MIRANDA, C.L., DEINZER, M.L., GRIFFIN, D.A. and HENDERSON, M.C. (1997). The regiospecific hydroxylation of lauric acid by rainbow trout (Oncorhynchus mykiss) cytochrome P450s. Drug Metab. Dispos. 25, 1176-1183.

SCHLENK, S., EL-ALFY, A. and BUHLER, D.R. (1997). Down regulation of hepatic flavin- containing monooxygenase activity by 17 -estradiol in rainbow trout (Oncorhynchus mykiss). Comp. Biochem. Physiol. 118C, 199-202.

MIRANDA, C.L., HENDERSON, M.C. and BUHLER, D.R. (1998). Evaluation of chemicals as inhibitors of trout cytochrome P450s. Toxicol. Appl. Pharmacol. 148, 237-244.

COK, I., WANG-BUHLER, J.-L., KEDZIERSKI, M.M., MIRANDA, C.L., YANG, Y.-H. and BUHLER, D.R. (1998). Expression of CYP2M1, CYP2K1 and CYP3A27 in brain , blood, small intestine and other tissues of rainbow trout. Biochem. Biophys. Res. Commun.244, 790-795.

YANG, Y.-H., WANG, J.-L., MIRANDA, C.L. and BUHLER, D.R. (1998). CYP2M1: cloning, sequencing and expression of a new cytochrome P450 from rainbow trout liver with fatty acid ( -6)-hydroxylation activity. Arch. Biochem. Biophys. 352, 271-280.

WILLIAMS, D.E., LECH, J.J. and BUHLER, D.R. (1998). Xenobiotics and xenoestrogens in fish: Modulation of cytochrome P450 and carcinogenesis. Mutation Res. 399, 179-192.

HAASCH, M.L., HENDERSON, M.C. and BUHLER, D.R. (1998). Induction of lauric acid hydroxylase activity in catfish and bluegill by peroxisome proliferating agents. Compr. Biochem. Physiol. 121C, 297-303.

HAASCH, M.L., HENDERSON, M.C. and BUHLER, D.R. (1998). Induction of CYP2M1 and CYP2K1 lauric acid hydroxylases activities by peroxisome proliferating agents in certain fish species: Possible implications. Mar. Environ. Res. 46, 37-40.

BUHLER, D.R. and WANG-BUHLER, J.-L. (1998). Rainbow trout cytochrome P450s: Purification, molecular aspects, metabolic activity, induction and role in environmental monitoring. Comp. Biochem. Physiol. Part C 121, 107-137.

LEE, S.-J., WANG-BUHLER, J.-L., COK, I., YU, T.S., YANG, Y.-H., MIRANDA, C.L., LECH, J.J. and BUHLER, D.R. (1998). Cloning, sequencing and tissue expression of CYP3A27, a new member of the CYP3A subfamily from embryonic and adult rainbow trout livers. Arch. Biochem. Biophys. 360, 53-61.

DONOHOE, R.M., WANG-BUHLER, J.-L., BUHLER, D.R. and CURTIS, L.R. (1999). Comparative response of cytochrome P4501A and estrogen-induced vitellogenesis to 3,3',4,4',5,5'-hexachlorobiphenyl in rainbow trout (Oncorhynchus mykiss). Environ. Toxicol. and Chem. 18, 1046-1052.

BUHLER, D.R., MIRANDA, C.L., HENDERSON, M.C., YANG, Y.-H., LEE, S.-J., and WANG- BUHLER, J.-L. (2000) Effects of 17 -estradiol and testosterone on hepatic mRNA/protein levels and catalytic activities of CYP2K1, CYP2M1 and CYP3A27 in rainbow trout (Oncorhynchus mykiss). Toxicol. Appl. Pharmacol. 168, 91-101.

YANG, Y.-H., MIRANDA, C. L., HENDERSON, M.C., WANG-BUHLER, J.-L. and BUHLER, D.R. (2000). Heterologous expression of CYP2K1 and identification of the expressed protein (BV-CYP2K1) as lauric acid ( -1) hydroxylase and aflatoxin B1 exo-epoxidase. Drug Metab. Dispos. 28, 1279-1283.