ZFIN ID: ZDB-PERS-040525-1
Sood, Raman
Email: rsood@mail.nih.gov
URL: http://www.genome.gov/10000299
Affiliation: Sood Lab
Address: National Human Genome Research Group The National Institutes of Health 6 Center Drive Building 6, Room B139A Bethesda, MD 20892 USA
Country: United States
Phone: 301-435-5746
Fax: 301-480-7848


Carrington, B., Sood, R. (2023) Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish. Bio-protocol. 13:e4732e4732
Simpson, C.L., Kimble, D.C., Chandrasekharappa, S.C., NISC Comparative Sequencing Program, Alqosayer, K., Holzinger, E., Carrington, B., McElderry, J., Sood, R., Al-Souqi, G., Albacha-Hejazi, H., Bailey-Wilson, J.E. (2023) A novel de novo TP63 mutation in whole-exome sequencing of a Syrian family with Oral cleft and ectrodactyly. Molecular genetics & genomic medicine. 11(8):e2179
Carrington, B., Ramanagoudr-Bhojappa, R., Bresciani, E., Han, T.U., Sood, R. (2022) A robust pipeline for efficient knock-in of point mutations and epitope tags in zebrafish using fluorescent PCR based screening. BMC Genomics. 23:810810
Weinstein, R., Bishop, K., Broadbridge, E., Yu, K., Carrington, B., Elkahloun, A., Zhen, T., Pei, W., Burgess, S.M., Liu, P., Bresciani, E., Sood, R. (2022) Zrsr2 Is Essential for the Embryonic Development and Splicing of Minor Introns in RNA and Protein Processing Genes in Zebrafish. International Journal of Molecular Sciences. 23(18)
Imani, J., Bodine, S.P.M., Lamattina, A.M., Ma, D.D., Shrestha, S., Maynard, D.M., Bishop, K., Nwokeji, A., Malicdan, M.C.V., Testa, L.C., Sood, R., Stump, B., Rosas, I.O., Perrella, M.A., Handin, R., Young, L.R., Gochuico, B.R., El-Chemaly, S. (2022) Dysregulated myosin in Hermansky-Pudlak syndrome lung fibroblasts is associated with increased cell motility. Respiratory research. 23:167
Carrington, B., Bishop, K., Sood, R. (2022) A Comprehensive Review of Indel Detection Methods for Identification of Zebrafish Knockout Mutants Generated by Genome-Editing Nucleases. Genes. 13(5)
Bresciani, E., Carrington, B., Yu, K., Kim, E.M.K., Zhen, T., Guzman, V.S., Broadbridge, E., Bishop, K., Kirby, M., Harper, U., Wincovitch, S., Dell'Orso, S., Sartorelli, V., Sood, R., Liu, P.P. (2021) Redundant mechanisms driven independently by RUNX1 and GATA2 for hematopoietic development. Blood advances. 5(23):4949-4962
Shin, U., Nakhro, K., Oh, C.K., Carrington, B., Song, H., Varshney, G.K., Kim, Y., Song, H., Jeon, S., Robbins, G., Kim, S., Yoon, S., Choi, Y.J., Kim, Y.J., Burgess, S., Kang, S., Sood, R., Lee, Y., Myung, K. (2021) Large-scale generation and phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes. DNA repair. 107:103173
Burke, E.A., Sturgeon, M., Zastrow, D.B., Fernandez, L., Prybol, C., Marwaha, S., Frothingham, E.P., Ward, P.A., Eng, C.M., Fresard, L., Montgomery, S.B., Enns, G.M., Fisher, P.G., Wolfe, L.A., Harding, B., Carrington, B., Bishop, K., Sood, R., Huang, Y., Elkahloun, A., Toro, C., Bassuk, A.G., Wheeler, M.T., Markello, T.C., Gahl, W.A., Malicdan, M.C.V. (2021) Compound heterozygous KCTD7 variants in progressive myoclonus epilepsy. Journal of neurogenetics. 35(2):74-83
Hong, S., Hu, P., Jang, J.H., Carrington, B., Sood, R., Berger, S.I., Roessler, E., Muenke, M. (2020) Functional analysis of Sonic Hedgehog variants associated with holoprosencephaly in humans using a CRISPR/Cas9 zebrafish model. Human Mutation. 41(12):2155-2166
Nagai-Tanima, M., Hong, S., Hu, P., Carrington, B., Sood, R., Roessler, E., Muenke, M. (2020) Rare hypomorphic human variation in the heptahelical domain of SMO contributes to holoprosencephaly phenotypes. Human Mutation. 41(12):2105-2118
Carrington, B., Weinstein, R.N., Sood, R. (2020) BE4max and AncBE4max Are Efficient in Germline Conversion of C:G to T:A Base Pairs in Zebrafish. Cells. 9(7):
Sloan, J.L., Achilly, N.P., Arnold, M.L., Catlett, J.L., Blake, T., Bishop, K., Jones, M., Harper, U., English, M.A., Anderson, S., Trivedi, N.S., Elkahloun, A., Hoffmann, V., Brooks, B.P., Sood, R., Venditti, C.P. (2020) The vitamin B12 processing enzyme, mmachc, is essential for zebrafish survival, growth and retinal morphology. Human molecular genetics. 29(13):2109-2123
Han, C.R., Holmsen, E., Carrington, B., Bishop, K., Zhu, Y.J., Starost, M., Meltzer, P., Sood, R., Liu, P., Cheng, S.Y. (2020) Generation of novel genetic models to dissect resistance to thyroid hormone receptor alpha in zebrafish. Thyroid : official journal of the American Thyroid Association. 30(2):314-328
Rissone, A., Jimenez, E., Bishop, K., Carrington, B., Slevin, C., Wincovitch, S.M., Sood, R., Candotti, F., Burgess, S.M. (2019) A model for reticular dysgenesis shows impaired sensory organ development and hair cell regeneration linked to cellular stress. Disease models & mechanisms. 12(12):
McElderry, J., Carrington, B., Bishop, K., Kim, E., Pei, W., Chen, Z., Ramanagoudr-Bhojappa, R., Prakash, A., Burgess, S.M., Liu, P.P., Sood, R. (2019) Splicing factor DHX15 affects tp53 and mdm2 expression via alternate splicing and promoter usage. Human molecular genetics. 28(24):4173-4185
Paul, C.D., Bishop, K., Devine, A., Paine, E.L., Staunton, J.R., Thomas, S.M., Thomas, J.R., Doyle, A.D., Miller Jenkins, L.M., Morgan, N.Y., Sood, R., Tanner, K. (2019) Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish. Cell systems. 9(2):187-206.e16
Paul, C.D., Devine, A., Bishop, K., Xu, Q., Wulftange, W.J., Burr, H., Daly, K.M., Lewis, C., Green, D.S., Staunton, J.R., Choksi, S., Liu, Z.G., Sood, R., Tanner, K. (2019) Human macrophages survive and adopt activated genotypes in living zebrafish. Scientific Reports. 9:1759
Pei, W., Xu, L., Huang, S.C., Pettie, K., Idol, J., Rissone, A., Jimenez, E., Sinclair, J.W., Slevin, C., Varshney, G.K., Jones, M., Carrington, B., Bishop, K., Huang, H., Sood, R., Lin, S., Burgess, S.M. (2018) Guided genetic screen to identify genes essential in the regeneration of hair cells and other tissues. NPJ Regenerative medicine. 3:11
Giannelou, A., Wang, H., Zhou, Q., Park, Y.H., Abu-Asab, M.S., Ylaya, K., Stone, D.L., Sediva, A., Sleiman, R., Sramkova, L., Bhatla, D., Serti, E., Tsai, W.L., Yang, D., Bishop, K., Carrington, B., Pei, W., Deuitch, N., Brooks, S., Edwan, J.H., Joshi, S., Prader, S., Kaiser, D., Owen, W.C., Sonbul, A.A., Zhang, Y., Niemela, J.E., Burgess, S.M., Boehm, M., Rehermann, B., Chae, J., Quezado, M.M., Ombrello, A.K., Buckley, R.H., Grom, A.A., Remmers, E.F., Pachlopnik, J.M., Su, H.C., Gutierrez-Cruz, G., Hewitt, S.M., Sood, R., Risma, K., Calvo, K.R., Rosenzweig, S.D., Gadina, M., Hafner, M., Sun, H.W., Kastner, D.L., Aksentijevich, I. (2018) Aberrant tRNA processing causes an autoinflammatory syndrome responsive to TNF inhibitors. Annals of the rheumatic diseases. 77(4):612-619
Justice, C.M., Kim, J., Kim, S.D., Kim, K., Yagnik, G., Cuellar, A., Carrington, B., Lu, C.L., Sood, R., Boyadjiev, S.A., Wilson, A.F. (2017) A variant associated with sagittal nonsyndromic craniosynostosis alters the regulatory function of a non-coding element. American journal of medical genetics. Part A. 173(11):2893-2897
Zhang, Y., Huang, H., Zhao, G., Yokoyama, T., Vega, H., Huang, Y., Sood, R., Bishop, K., Maduro, V., Accardi, J., Toro, C., Boerkoel, C.F., Lyons, K., Gahl, W.A., Duan, X., Malicdan, M.C., Lin, S. (2017) ATP6V1H Deficiency Impairs Bone Development through Activation of MMP9 and MMP13. PLoS Genetics. 13:e1006481
Varshney, G.K., Carrington, B., Pei, W., Bishop, K., Chen, Z., Fan, C., Xu, L., Jones, M., LaFave, M.C., Ledin, J., Sood, R., Burgess, S.M. (2016) A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish. Nature Protocols. 11:2357-2375
Pei, W., Xu, L., Varshney, G.K., Carrington, B., Bishop, K., Jones, M., Huang, S.C., Idol, J., Pretorius, P.R., Beirl, A., Schimmenti, L.A., Kindt, K.S., Sood, R., Burgess, S.M. (2016) Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases. Scientific Reports. 6:29946
Justice, C.M., Bishop, K., Carrington, B., Mullikin, J.C., Swindle, K., Marosy, B., Sood, R., Miller, N.H., Wilson, A.F. (2016) Evaluation of IRX Genes and Conserved Non-coding Elements in a Region on 5p13.3 Linked to Families with Familial Idiopathic Scoliosis and Kyphosis. G3 (Bethesda). 6(6):1707-12
Varshney, G.K., Zhang, S., Pei, W., Adomako-Ankomah, A., Fohtung, J., Schaffer, K., Carrington, B., Maskeri, A., Slevin, C., Wolfsberg, T., Ledin, J., Sood, R., Burgess, S.M. (2016) CRISPRz: a database of zebrafish validated sgRNAs. Nucleic acids research. 44(D1):D822-6
Carrington, B., Varshney, G.K., Burgess, S.M., Sood, R. (2015) CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity. Nucleic acids research. 43(22):e157
Rissone, A., Weinacht, K.G., la Marca, G., Bishop, K., Giocaliere, E., Jagadeesh, J., Felgentreff, K., Dobbs, K., Al-Herz, W., Jones, M., Chandrasekharappa, S., Kirby, M., Wincovitch, S., Simon, K.L., Itan, Y., DeVine, A., Schlaeger, T., Schambach, A., Sood, R., Notarangelo, L.D., Candotti, F. (2015) Reticular dysgenesis-associated AK2 protects hematopoietic stem and progenitor cell development from oxidative stress. The Journal of experimental medicine. 212(8):1185-202
Varshney, G.K., Pei, W., LaFave, M.C., Idol, J., Xu, L., Gallardo, V., Carrington, B., Bishop, K., Jones, M., Li, M., Harper, U., Huang, S.C., Prakash, A., Chen, W., Sood, R., Ledin, J., Burgess, S.M. (2015) High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9. Genome research. 25(7):1030-42
Varshney, G.K., Sood, R., Burgess, S.M. (2015) Understanding and Editing the Zebrafish Genome. Advances in genetics. 92:1-52
Hao, H., Veleri, S., Sun, B., Kim, D.S., Keeley, P.W., Kim, J.W., Yang, H.J., Yadav, S.P., Manjunath, S.H., Sood, R., Liu, P., Reese, B.E., Swaroop, A. (2014) Regulation of a novel isoform of Receptor Expression Enhancing Protein REEP6 in rod photoreceptors by bZIP transcription factor NRL. Human molecular genetics. 23(16):4260-71
Bresciani, E., Carrington, B., Wincovitch, S., Jones, M., Gore, A.V., Weinstein, B.M., Sood, R., Liu, P.P. (2014) CBFβ and RUNX1 are required at 2 different steps during the development of hematopoietic stem cells in zebrafish. Blood. 124(1):70-8
Zhou, Q., Yang, D., Ombrello, A.K., Zavialov, A.V., Toro, C., Zavialov, A.V., Stone, D.L., Chae, J.J., Rosenzweig, S.D., Bishop, K., Barron, K.S., Kuehn, H.S., Hoffmann, P., Negro, A., Tsai, W.L., Cowen, E.W., Pei, W., Milner, J.D., Silvin, C., Heller, T., Chin, D.T., Patronas, N.J., Barber, J.S., Lee, C.C., Wood, G.M., Ling, A., Kelly, S.J., Kleiner, D.E., Mullikin, J.C., Ganson, N.J., Kong, H.H., Hambleton, S., Candotti, F., Quezado, M.M., Calvo, K.R., Alao, H., Barham, B.K., Jones, A., Meschia, J.F., Worrall, B.B., Kasner, S.E., Rich, S.S., Goldbach-Mansky, R., Abinun, M., Chalom, E., Gotte, A.C., Punaro, M., Pascual, V., Verbsky, J.W., Torgerson, T.R., Singer, N.G., Gershon, T.R., Ozen, S., Karadag, O., Fleisher, T.A., Remmers, E.F., Burgess, S.M., Moir, S.L., Gadina, M., Sood, R., Hershfield, M.S., Boehm, M., Kastner, D.L., Aksentijevich, I. (2014) Early-onset stroke and vasculopathy associated with mutations in ADA2. The New England Journal of Medicine. 370(10):911-920
Vilboux, T., Lev, A., Malicdan, M.C., Simon, A.J., Järvinen, P., Racek, T., Puchalka, J., Sood, R., Carrington, B., Bishop, K., Mullikin, J., Huizing, M., Garty, B.Z., Eyal, E., Wolach, B., Gavrieli, R., Toren, A., Soudack, M., Atawneh, O.M., Babushkin, T., Schiby, G., Cullinane, A., Avivi, C., Polak-Charcon, S., Barshack, I., Amariglio, N., Rechavi, G., van der Werff ten Bosch, J., Anikster, Y., Klein, C., Gahl, W.A., and Somech, R. (2013) A congenital neutrophil defect syndrome associated with mutations in VPS45. New. Engl. J. Med.. 369(1):54-65
Sood, R., Carrington, B., Bishop, K., Jones, M., Rissone, A., Candotti, F., Chandrasekharappa, S.C., and Liu, P. (2013) Efficient Methods for Targeted Mutagenesis in Zebrafish Using Zinc-Finger Nucleases: Data from Targeting of Nine Genes Using CompoZr or CoDA ZFNs. PLoS One. 8(2):e57239
Cunningham, L., Finckbeiner, S., Hyde, R.K., Southall, N., Marugan, J., Yedavalli, V.R., Dehdashti, S.J., Reinhold, W.C., Alemu, L., Zhao, L., Yeh, J.R., Sood, R., Pommier, Y., Austin, C.P., Jeang, K.T., Zheng, W., and Liu, P. (2012) Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBFbeta interaction. Proceedings of the National Academy of Sciences of the United States of America. 109(36):14592-14597
English, M.A., Lei, L., Blake, T., Wincovitch, S.M., Sood, R., Azuma, M., Hickstein, D., and Paul Liu, P. (2012) Incomplete splicing, cell division defects, and hematopoietic blockage in dhx8 mutant zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 241(5):879-889
Sood, R., and Liu, P. (2012) Novel Insights into the Genetic Controls of Primitive and Definitive Hematopoiesis from Zebrafish Models. Advances in hematology. 2012:830703
Schoenebeck, J.J., Hutchinson, S.A., Byers, A., Beale, H.C., Carrington, B., Faden, D.L., Rimbault, M., Decker, B., Kidd, J.M., Sood, R., Boyko, A.R., Fondon, J.W., Wayne, R.K., Bustamante, C.D., Ciruna, B., and Ostrander, E.A. (2012) Variation of BMP3 Contributes to Dog Breed Skull Diversity. PLoS Genetics. 8(8):e1002849
Veleri, S., Bishop, K., Dalle Nogare, D.E., English, M.A., Foskett, T.J., Chitnis, A., Sood, R., Liu, P., and Swaroop, A. (2012) Knockdown of Bardet-Biedl Syndrome Gene BBS9/PTHB1 Leads to Cilia Defects. PLoS One. 7(3):e34389
Finckbeiner, S., Ko, P.J., Carrington, B., Sood, R., Gross, K., Dolnick, B., Sufrin, J., and Liu, P. (2011) Transient Knockdown and Overexpression Reveal a Developmental Role for the Zebrafish enosf1b Gene. Cell & Bioscience. 1:32
Shive, H.R., West, R.R., Embree, L.J., Azuma, M., Sood, R., Liu, P., and Hickstein, D.D. (2010) brca2 in zebrafish ovarian development, spermatogenesis, and tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America. 107(45):19350-19355
Pei, W., Kratz, L.E., Bernardini, I., Sood, R., Yokogawa, T., Dorward, H., Ciccone, C., Kelley, R.I., Anikster, Y., Burgess, H.A., Huizing, M., and Feldman, B. (2010) A model of Costeff Syndrome reveals metabolic and protective functions of mitochondrial OPA3. Development (Cambridge, England). 137(15):2587-2596
Sood, R., English, M.A., Belele, C.L., Jin, H., Bishop, K., Haskins, R., McKinney, M.C., Chahal, J., Weinstein, B.M., Wen, Z., and Liu, P.P. (2010) Development of multilineage adult hematopoiesis in the zebrafish with a runx1 truncation mutation. Blood. 115(14):2806-2809
Belele, C.L., English, M.A., Chahal, J., Burnetti, A., Finckbeiner, S.M., Gibney, G., Kirby, M., Sood, R., and Liu, P. (2009) Differential requirement for Gata1 DNA binding and transactivation between primitive and definitive stages of hematopoiesis in zebrafish. Blood. 114(25):5162-5172
Jin, H., Sood, R., Xu, J., Zhen, F., English, M.A., Liu, P.P., and Wen, Z. (2009) Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI. Development (Cambridge, England). 136(4):647-654
Sood, R., English, M.A., Jones, M., Mullikin, J., Wang, D.M., Anderson, M., Wu, D., Chandrasekharappa, S.C., Yu, J., Zhang, J., and Liu, P.P. (2006) Methods for reverse genetic screening in zebrafish by resequencing and TILLING. Methods (San Diego, Calif.). 39(3):220-227