|ZFIN ID: ZDB-PERS-010221-4|
BIOGRAPHY AND RESEARCH INTERESTS
My research focuses on the formation of the primitive heart tube. Specifically, I am interested in factors controlling the migration of the precardiac mesoderm from bilateral positions to the embryonic midline. I am using two mutations, miles apart and two of hearts, to study this process. miles apart encodes the zebrafish orthologue of the mammalian G protein-coupled receptor S1P2 and seems to function with the, as of yet unidentified, two of hearts locus to regulate precardiac mesoderm migration.
Balczerski, B., Matsutani, M., Castillo, P., Osborne, N., Stainier, D.Y., and Crump, J.G. (2012) Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors. Developmental Biology. 361(2):230-241
Osborne, N., Brand-Arzamendi, K., Ober, E.A., Jin, S.W., Verkade, H., Holtzman, N.G., Yelon, D., and Stainier, D.Y. (2008) The spinster homolog, two of hearts, is required for sphingosine 1-phosphate signaling in zebrafish. Current biology : CB. 18(23):1882-1888
Osborne, N.F. (2005) Sphingosine 1-phosphate signaling in zebrafish heart and endoderm development. Ph.D. Thesis. :133p
Kupperman, E., An, S., Osborne, N., Waldron, S., and Stainier, D.Y. (2000) A sphingosine-1-phosphate receptor regulates cell migration during vertebrate heart development. Nature. 406(6792):192-195
Pogliano J, Osborne N, Sharp MD, Abanes-De Mello A, Perez A, Sun YL, Pogliano K. A vital stain for studying membrane dynamics in bacteria: a novel mechanism controlling septation during Bacillus subtilis sporulation. Mol Microbiol. 1999 Feb;31(4):1149-59.