header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-LAB-970501-1
Dowling Lab
PI/Director: Dowling, John E.
Contact Person:
Email:
URL:
Address: Department of Molecular and Cellular Biology Harvard University 16 Divinity Avenue Cambridge, MA 02138 USA
Country: United States
Phone:
Fax:
Line Designation: a


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 22 genomic features


STATEMENT OF RESEARCH INTERESTS
Our current interests are focused in two directions. First, we have developed behavioral tests to isolate visual system specific mutations from chemically-mutagenized zebrafish. Both recessive and dominant mutations that affect the retina have been isolated and are currently being analyzed histologically, electrophysiologically and biochemically. Ongoing screening is continually revealing new mutants. A second interest is the molecular basis of retinal development. In particular, the effects of retinoic acid on candidate genes involved in early eye and photoreceptor development are being explored as well as detailed examination of early retinal development in zebrafish.


LAB MEMBERS


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
Dowling, J.E. (2018) A Life in Vision. Annual review of vision science. 4:1-23
Moyano, M., Porteros, A., and Dowling, J.E. (2013) The effects of nicotine on cone and rod b-wave responses in larval zebrafish. Visual neuroscience. 28:1-5
Darland, T., Mauch, J.T., Meier, E.M., Hagan, S.J., Dowling, J.E., and Darland, D.C. (2012) Sulpiride, but not SCH23390, modifies cocaine-induced conditioned place preference and expression of tyrosine hydroxylase and elongation factor 1α in zebrafish. Pharmacology, biochemistry, and behavior. 103(2):157-167
Li, Y.N., and Dowling, J.E. (2012) Bipolar cell-photoreceptor connectivity in the zebrafish (Danio rerio) retina. The Journal of comparative neurology. 520(16):3786-3802
Le, H.G., Dowling, J.E., and Cameron, D.J. (2012) Early retinoic acid deprivation in developing zebrafish results in microphthalmia. Visual neuroscience. 29(4-5):219-228
Dowling, J.E. (2012) What can a zebrafish see with only an off-pathway and other fish stories. Journal of Ophthalmic & Vision Research. 7(1):97-99
Hensley, M.R., Emran, F., Bonilla, S., Zhang, L., Zhong, W., Grosu, P., Dowling, J.E., and Leung, Y.F. (2011) Cellular Expression of Smarca4 (Brg1)-regulated Genes in Zebrafish Retinas. BMC Developmental Biology. 11(1):45
Stujenske, J.M., Dowling, J.E., and Emran, F. (2011) The Bugeye Mutant Zebrafish Exhibits Visual Deficits that Arise with the Onset of an Enlarged Eye Phenotype. Investigative ophthalmology & visual science. 52(7):4200-4207
Emran, F., and Dowling, J.E. (2010) Larval zebrafish turn off their photoreceptors at night. Communicative & integrative biology. 3(5):430-432
Emran, F., Rihel, J., Adolph, A.R., and Dowling, J.E. (2010) Zebrafish larvae lose vision at night. Proceedings of the National Academy of Sciences of the United States of America. 107(13):6034-6039
Li, Y.N., Matsui, J.I., and Dowling, J.E. (2009) Specificity of the horizontal cell-photoreceptor connections in the zebrafish (Danio rerio) retina. The Journal of comparative neurology. 516(5):442-453
Emran, F., Rihel, J., and Dowling, J.E. (2008) A behavioral assay to measure responsiveness of zebrafish to changes in light intensities. Journal of visualized experiments : JoVE. (20)
Leung, Y.F., Ma, P., Link, B.A., and Dowling, J.E. (2008) Factorial microarray analysis of zebrafish retinal development. Proceedings of the National Academy of Sciences of the United States of America. 105(35):12909-12914
Kojima, D., Dowling, J.E., and Fukada, Y. (2008) Probing Pineal-specific Gene Expression with Transgenic Zebrafish. Photochemistry and photobiology. 84(4):1011-1015
Kojima, D., Torii, M., Fukada, Y., and Dowling, J.E. (2008) Differential expression of duplicated VAL-opsin genes in the developing zebrafish. Journal of neurochemistry. 104(5):1364-1371
Song, P.I., Matsui, J.I., and Dowling, J.E. (2008) Morphological types and connectivity of horizontal cells found in the adult zebrafish (Danio rerio) retina. The Journal of comparative neurology. 506(2):328-338
Fadool, J.M., and Dowling, J.E. (2008) Zebrafish: A model system for the study of eye genetics. Progress in Retinal and Eye Research. 27(1):89-110
Emran, F., Rihel, J., Adolph, A.R., Wong, K.Y., Kraves, S., and Dowling, J.E. (2007) OFF ganglion cells cannot drive the optokinetic reflex in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 104(48):19126-19131
Leung, Y.F., Ma, P., and Dowling, J.E. (2007) Gene expression profiling of zebrafish embryonic retinal pigment epithelium in vivo. Investigative ophthalmology & visual science. 48(2):881-890
Matsui, J.I., Egana, A.L., Sponholtz, T.R., Adolph, A.R., and Dowling, J.E. (2006) Effects of ethanol on photoreceptors and visual function in developing zebrafish. Investigative ophthalmology & visual science. 47(10):4589-4597
Perkins, B.D., Nicholas, C.S., Baye, L.M., Link, B.A., and Dowling, J.E. (2005) dazed gene is necessary for late cell type development and retinal cell maintenance in the zebrafish retina. Developmental dynamics : an official publication of the American Association of Anatomists. 233(2):680-694
Gross, J.M., Perkins, B.D., Amsterdam, A., Egana, A., Darland, T., Matsui, J.I., Sciascia, S., Hopkins, N., and Dowling, J.E. (2005) Identification of Zebrafish Insertional Mutants with Defects in Visual System Development and Function. Genetics. 170(1):245-261
Gross, J.M., and Dowling, J.E. (2005) Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina. Proceedings of the National Academy of Sciences of the United States of America. 102(12):4371-4376
Leung, Y.F., and Dowling, J.E. (2005) Gene Expression Profiling of Zebrafish Embryonic Retina. Zebrafish. 2(4):269-283
Qian, H., Zhu, Y., Ramsey, D.J., Chappell, R.L., Dowling, J.E., and Ripps, H. (2005) Directional Asymmetries in the Optokinetic Response of Larval Zebrafish (Danio rerio). Zebrafish. 2(3):189-196
Perkins, B.D., Fadool, J.M., and Dowling, J.E. (2004) Photoreceptor structure and development: analyses using GFP transgenes. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:315-331
Wong, K.Y., Gray, J. Hayward, C.J.C., Adolph, A.R., and Dowling, J.E. (2004) Glutamatergic Mechanisms in the Outer Retina of Larval Zebrafish: Analysis of Electroretinogram b- and d-Waves Using a Novel Preparation. Zebrafish. 1(2):121-131
Kainz, P.M., Adolph, A.R., Wong, K.Y., and Dowling, J.E. (2003) Lazy eyes zebrafish mutation affects Müller glial cells, compromising photoreceptor function and causing partial blindness. The Journal of comparative neurology. 463(3):265-280
Gregg, R.G., Willer, G.B., Fadool, J.M., Dowling, J.E., and Link, B.A. (2003) Positional cloning of the young mutation identifies an essential role for the Brahma chromatin remodeling complex in mediating retinal cell differentiation. Proceedings of the National Academy of Sciences of the United States of America. 100(11):6535-6540
Mangrum, W.I., Dowling, J.E., and Cohen, E.D. (2002) A morphological classification of ganglion cells in the zebrafish retina. Visual neuroscience. 19(6):767-779
Perkins, B.D., Kainz, P.M., O'Malley, D.M., and Dowling, J.E. (2002) Transgenic expression of a GFP-rhodopsin COOH-terminal fusion protein in zebrafish rod photoreceptors. Visual neuroscience. 19(3):257-264
Darland, T. and Dowling, J.E. (2001) Behavioral screening for cocaine sensitivity in mutagenized zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 98(20):11691-11696
Link, B.A., Kainz, P.M., Ryou, T., and Dowling, J.E. (2001) The perplexed and confused mutations affect distinct stages during the transition from proliferating to post-mitotic cells within the zebrafish retina. Developmental Biology. 236(2):436-453
Allwardt, B.A. and Dowling, J.E. (2001) The pineal gland in wild-type and two zebrafish mutants with retinal defects. Journal of neurocytology. 30(6):493-501
Allwardt, B.A., Lall, A.B., Brockerhoff, S.E., and Dowling, J.E. (2001) Synapse formation is arrested in retinal photoreceptors of the zebrafish nrc mutant. The Journal of neuroscience : the official journal of the Society for Neuroscience. 21(7):2330-2342
Kainz, P.M. and Dowling, J.E. (2001) A novel photoreceptor cell mutation in zebrafish. Investigative ophthalmology & visual science. 42(4):4096
Link, B.A., Darland, T., and Dowling, J.E. (2001) Isolation of zebrafish mutations that affect the development and maintenance of the lens. Investigative ophthalmology & visual science. 42(4):2882
Perkins, B.D. and Dowling, J.E. (2001) Photoreceptor-specific expression of GFP in the zebrafish, Danio rerio. Investigative ophthalmology & visual science. 42(4):1953
Dowling, J.E., Kolb, H., Ripps, H., and Wu, S.M.-S. (2001) Concepts and challenges in retinal biology. Amsterdam; New York: Elsevier. :742P
Peterson, R.T., Link, B.A., Dowling, J.E., and Schreiber, S.L. (2000) Small molecule developmental screens reveal the logic and timing of vertebrate development. Proceedings of the National Academy of Sciences of the United States of America. 97(24):12965-12969
Link, B.A., Fadool, J.M., Malicki, J., and Dowling, J.E. (2000) The zebrafish young mutation acts non-cell-autonomously to uncouple differentiation from specification for all retinal cells. Development (Cambridge, England). 127(10):2177-2188
Li, L. and Dowling, J.E. (2000) Effects of dopamine depletion on visual sensitivity of zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(5):1893-903
Li, L. and Dowling, J.E. (2000) Disruption of the olfactoretinal centrifugal pathway may relate to the visual system defect in night blindness b mutant zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(5):1883-1892
Wong, K.Y., Cohen, E.D., Adolph, A.R., and Dowling, J.E. (2000) The d-wave of the zebrafish ERG derives mainly from OFF-bipolar cell activity. Investigative ophthalmology & visual science. 41(4):3305
Schmitt, E.A., Hyatt, G.A., and Dowling, J.E. (1999) Erratum: Temporal and spatial patterns of opsin gene expression in the zebrafish (Danio rerio): corrections with additions. Visual neuroscience. 16(3):601-605
Schmitt, E.A. and Dowling, J.E. (1999) Early retinal development in the zebrafish, Danio rerio: light and electron microscopic analysis. The Journal of comparative neurology. 404:515-536
Li, L. and Dowling, J.E. (1998) Zebrafish visual sensitivity is regulated by a circadian clock. Visual neuroscience. 15:851-857
Brockerhoff, S.E., Dowling, J.E., and Hurley, J.B. (1998) Zebrafish retinal mutants. Vision Research. 38:1335-1339
Fadool, J.M., Hartl, D.L., and Dowling, J.E. (1998) Transposition of the mariner element from Drosophila mauritiana in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 95:5182-5186
Connaughton, V.P. and Dowling, J.E. (1998) Comparative morphology of distal neurons in larval and adult zebrafish retinas. Vision Research. 38:13-18
Dowling, J. (1998) Searching for visual system mutants in zebrafish. In Physiological and Molecular Mechanisms of Visual Function. Third Great Basin Visual Science Symposium, Salt Lake City: Eccles Ophthalmic Information Resource, University of Utah.
Li, L. and Dowling, J.E. (1997) A dominant form of inherited retinal degeneration caused by a non-photoreceptor cell-specific mutation. Proceedings of the National Academy of Sciences of the United States of America. 94:11645-11650
Brockerhoff, S.E., Hurley, J.B., Niemi, G.A., and Dowling, J.E. (1997) A new form of inherited red-blindness identified in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 17(11):4236-4242
Fulwiler, C., Schmitt, E.A., Kim, J.M.J., and Dowling, J.E. (1997) Retinal patterning in the zebrafish mutant Cyclops. The Journal of comparative neurology. 381(4):449-460
Fadool, J.M., Brockerhoff, S.E., Hyatt, G.A., and Dowling, J.E. (1997) Mutations affecting eye morphology in the developing zebrafish (Danio rerio). Developmental genetics. 20(3):288-295
Hyatt, G.A., Schmitt, E.A., Fadool, J.M., and Dowling, J.E. (1996) Retinoic acid alters photoreceptor development in vivo. Proceedings of the National Academy of Sciences of the United States of America. 93(23):13298-13303
Schmitt, E.A. and Dowling, J.E. (1996) Comparison of topographical patterns of ganglion and photoreceptor cell differentiation in the retina of the zebrafish, Danio rerio. The Journal of comparative neurology. 371(2):222-234
Schmitt, E.A., Fadool, J.M., and Dowling, J.E. (1996) Zebrafish ultraviolet cone opsin. Investigative ophthalmology & visual science. 37:695
Hyatt, G.A., Schmitt, E.A., Marsh-Armstrong, N., McCaffery, P., Dräger, U.C., and Dowling, J.E. (1996) Retinoic acid establishes ventral retinal characteristics. Development (Cambridge, England). 122:195-204
Brockerhoff, S.E., Hurley, J.B., Janssen-Bienhold, U., Neuhauss, S.C.F., Driever, W., and Dowling, J.E. (1995) A behavioral screen for isolating zebrafish mutants with visual system defects. Proceedings of the National Academy of Sciences of the United States of America. 92:10545-10549
Robinson, J., Schmitt, E.A., and Dowling, J.E. (1995) Temporal and spatial patterns of opsin gene expression in zebrafish (Danio rerio). Visual neuroscience. 12:895-906
Marsh-Armstrong, N., McCaffery, P., Hyatt, G., Alonso, L., Dowling, J.E., Gilbert, W., and Dräger, U.C. (1995) Retinoic acid in the anteroposterior patterning of the zebrafish trunk. Roux's archives of developmental biology : the official organ of the EDBO. 205:103-113
Marsh-Armstrong, N., McCaffery, P., Gilbert, W., Dowling, J.E., and Dräger, U.C. (1994) Retinoic acid is necessary for development of the ventral retina in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 91:7286-7290
Schmitt, E.A. and Dowling, J.E. (1994) Early eye morphogenesis in the zebrafish, Brachydanio rerio. The Journal of comparative neurology. 344:532-542
Robinson, J., Schmitt, E.A., Hárosi, F.I., Reece, R.J., and Dowling, J.E. (1993) Zebrafish ultraviolet visual pigment: absorption spectrum, sequence, and localization. Proceedings of the National Academy of Sciences of the United States of America. 90:6009-6012
Hyatt, G.A., Schmitt, E.A., Marsh-Armstrong, N.R., and Dowling, J.E. (1992) Retinoic acid-induced duplication of the zebrafish retina. Proceedings of the National Academy of Sciences of the United States of America. 89:8293-8297
Fulwiler, C., Schmitt, E.A., and Dowling, J.E. (1991) Pattern regulation in the retina of the zebrafish mutant cyclops. Soc. Neurosci. Abstr.. 17:9
Schmitt, E.A., Fulwiler, C., Goodrich, L.V., and Dowling, J.E. (1991) Eye morphogenesis in the zebrafish. Soc. Neurosci. Abstr.. 17:9