header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-LAB-970418-14
Lieschke Laboratory
PI/Director: Lieschke, Graham J.
Contact Person: Lieschke, Graham J.
Email: Graham.Lieschke@monash.edu
URL:
Address: Australian Regenerative Medicine Institute Level 1, Building 75 Monash University, Wellington Road Clayton VIC 3800 Australia
Country: Australia
Phone: +61 3 9902 9720
Fax: +61 3 9902 9729
Line Designation: gl


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 40 genomic features


STATEMENT OF RESEARCH INTERESTS
The laboratory studies the molecular control of hematopoiesis, particularly granulopoiesis, both under normal physiological conditions and in malignancy. Particular projects focus on signalling from the cytokine receptors (particularly the G-CSF receptor), the structure/function of the G-CSF ligand-receptor interaction, and the role of endogenous cytokine production in the leukaemic phenotype. Our interest in granulopoiesis now includes the use of zebrafish as a reagent for genetic, molecular and cellular studies. Based on our characterisation of adult and embryonic myelopoiesis in zebrafish, we are undertaking screens for mutants with developmental myeloid failure.


LAB MEMBERS
Pazhakh, Vahid Post-Doc Zhang, Zuobing Post-Doc Manley, Harriet Graduate Student
De Seram, Mia Research Staff Varma, Sony Research Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
He, S., Manley, H.R. (2019) The 11th Zebrafish Disease Models Conference in Leiden, 2018. Zebrafish. 16(4):421-426
Pazhakh, V., Lieschke, G.J. (2019) Hematopoietic growth factors: the scenario in zebrafish. Growth factors (Chur, Switzerland). 36(5-6):196-212
Alifui-Segbaya, F., Bowman, J., White, A.R., Varma, S., Lieschke, G.J., George, R. (2018) Toxicological assessment of additively manufactured methacrylates for medical devices in dentistry. Acta biomaterialia. 78:64-77
Ellett, F., Pazhakh, V., Pase, L., Benard, E.L., Weerasinghe, H., Azabdaftari, D., Alasmari, S., Andrianopoulos, A., Lieschke, G.J. (2018) Macrophages protect Talaromyces marneffei conidia from myeloperoxidase-dependent neutrophil fungicidal activity during infection establishment in vivo. PLoS pathogens. 14:e1007063
Hall, C.J., Sanderson, L.E., Lawrence, L.M., Pool, B., van der Kroef, M., Ashimbayeva, E., Britto, D., Harper, J.L., Lieschke, G.J., Astin, J.W., Crosier, K.E., Dalbeth, N., Crosier, P.S. (2018) Blocking fatty acid-fueled mROS production within macrophages alleviates acute gouty inflammation. The Journal of Clinical Investigation. 128(5):1752-1771
Schmitz, U., Pinello, N., Jia, F., Alasmari, S., Ritchie, W., Keightley, M.C., Shini, S., Lieschke, G., Wong, J., Rasko, J. (2017) Intron retention enhances gene regulatory complexity in vertebrates. Genome biology. 18:216
Witzel, M., Petersheim, D., Fan, Y., Bahrami, E., Racek, T., Rohlfs, M., Puchałka, J., Mertes, C., Gagneur, J., Ziegenhain, C., Enard, W., Stray-Pedersen, A., Arkwright, P.D., Abboud, M.R., Pazhakh, V., Lieschke, G.J., Krawitz, P.M., Dahlhoff, M., Schneider, M.R., Wolf, E., Horny, H.P., Schmidt, H., Schäffer, A.A., Klein, C. (2017) Chromatin-remodeling factor SMARCD2 regulates transcriptional networks controlling differentiation of neutrophil granulocytes. Nature Genetics. 49(5):742-752
Keightley, M.C., Carradice, D.P., Layton, J.E., Pase, L., Bertrand, J.Y., Wittig, J.G., Dakic, A., Badrock, A.P., Cole, N.J., Traver, D., Nutt, S.L., McCoey, J., Buckle, A.M., Heath, J.K., Lieschke, G.J. (2017) The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger. Nature communications. 8:14911
Pazhakh, V., Clark, S., Keightley, M.C., Lieschke, G.J. (2017) A GCSFR/CSF3R zebrafish mutant models the persistent basal neutrophil deficiency of severe congenital neutropenia. Scientific Reports. 7:44455
Bhuiyan, M.S., Ellett, F., Murray, G.L., Kostoulias, X., Cerqueira, G.M., Schulze, K.E., Mahamad Maifiah, M.H., Li, J., Creek, D.J., Lieschke, G.J., Peleg, A.Y. (2016) Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis. Proceedings of the National Academy of Sciences of the United States of America. 113(34):9599-604
Keightley, M.C., Markmiller, S., Love, C.G., Rasko, J.E., Lieschke, G.J., Heath, J.K. (2016) Experimental approaches to studying the nature and impact of splicing variation in zebrafish. Methods in cell biology. 135:259-88
Keightley, M.C., Wang, C.H., Pazhakh, V., Lieschke, G.J. (2014) Delineating the roles of neutrophils and macrophages in zebrafish regeneration models. The international journal of biochemistry & cell biology. 56C:92-106
Nguyen, P.D., Hollway, G.E., Sonntag, C., Miles, L.B., Hall, T.E., Berger, S., Fernandez, K.J., Gurevich, D.B., Cole, N.J., Alaei, S., Ramialison, M., Sutherland, R.L., Polo, J.M., Lieschke, G.J., Currie, P.D. (2014) Haematopoietic stem cell induction by somite-derived endothelial cells controlled by meox1. Nature. 512(7514):314-8
Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M. (2014) Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton. Mechanisms of Development. 133:77-90
Markmiller, S., Cloonan, N., Lardelli, R.M., Doggett, K., Keightley, M.C., Boglev, Y., Trotter, A.J., Ng, A.Y., Wilkins, S.J., Verkade, H., Ober, E.A., Field, H.A., Grimmond, S.M., Lieschke, G.J., Stainier, D.Y., and Heath, J.K. (2014) Minor class splicing shapes the zebrafish transcriptome during development. Proceedings of the National Academy of Sciences of the United States of America. 111(8):3062-3067
Henry, K.M., Pase, L., Ramos-Lopez, C.F., Lieschke, G.J., Renshaw, S.A., and Reyes-Aldasoro, C.C. (2013) PhagoSight: An Open-Source MATLAB® Package for the Analysis of Fluorescent Neutrophil and Macrophage Migration in a Zebrafish Model. PLoS One. 8(8):e72636
Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Lieschke, G.J., Crosier, K.E., and Crosier, P.S. (2013) Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production. Cell Metabolism. 18(2):265-278
Keightley, M.C., Crowhurst, M.O., Layton, J.E., Beilharz, T., Markmiller, S., Varma, S., Hogan, B.M., de Jong-Curtain, T.A., Heath, J.K., and Lieschke, G.J. (2013) In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation. FEBS letters. 587(14):2150-2157
Boglev, Y., Badrock, A.P., Trotter, A.J., Du, Q., Richardson, E.J., Parslow, A.C., Markmiller, S.J., Hall, N.E., de Jong-Curtain, T.A., Ng, A.Y., Verkade, H., Ober, E.A., Field, H.A., Shin, D., Shin, C.H., Hannan, K.M., Hannan, R.D., Pearson, R.B., Kim, S.H., Ess, K.C., Lieschke, G.J., Stainier, D.Y., and Heath, J.K. (2013) Autophagy induction is a tor- and tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis. PLoS Genetics. 9(2):e1002379
Palha, N., Guivel-Benhassine, F., Briolat, V., Lutfalla, G., Sourisseau, M., Ellett, F., Wang, C.H., Lieschke, G.J., Herbomel, P., Schwartz, O., and Levraud, J.P. (2013) Real-time whole-body visualization of chikungunya virus infection and host interferon response in zebrafish. PLoS pathogens. 9(9):e1003619
Svahn, A.J., Graeber, M.B., Ellett, F., Lieschke, G.J., Rinkwitz, S., Bennett, M.R., and Becker, T.S. (2013) Development of ramified microglia from early macrophages in the zebrafish optic tectum. Developmental Neurobiology. 73(1):60-71
Pase, L., Layton, J.E., Wittmann, C., Ellett, F., Nowell, C.J., Reyes-Aldasoro, C.C., Varma, S., Rogers, K.L., Hall, C.J., Keightley, M.C., Crosier, P.S., Grabher, C., Heath, J.K., Renshaw, S.A., and Lieschke, G.J. (2012) Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish. Current biology : CB. 22(19):1818-1824
Goldshmit, Y., Matteo, R., Sztal, T., Ellett, F., Frisca, F., Moreno, K., Crombie, D., Lieschke, G.J., Currie, P.D., Sabbadini, R.A., and Pebay, A. (2012) Blockage of Lysophosphatidic Acid Signaling Improves Spinal Cord Injury Outcomes. The American journal of pathology. 181(3):978-992
Macheda, M.L., Sun, W.W., Kugathasan, K., Hogan, B.M., Bower, N.I., Halford, M.M., Zhang, Y.F., Jacques, B.E., Lieschke, G.J., Dabdoub, A., and Stacker, S.A. (2012) The Wnt Receptor Ryk Plays a Role in Mammalian Planar Cell Polarity Signaling. The Journal of biological chemistry. 287(35):29312-29323
Cai, H., Santiago, F.S., Prado-Lourenco, L., Wang, B., Patrikakis, M., Davenport, M.P., Maghzal, G.J., Stocker, R., Parish, C.R., Chong, B.H., Lieschke, G.J., Wong, T.W., Chesterman, C.N., Francis, D.J., Moloney, F.J., Barnetson, R.S., Halliday, G.M., and Khachigian, L.M. (2012) DNAzyme Targeting c-jun Suppresses Skin Cancer Growth. Science Translational Medicine. 4(139):139ra82
Benard, E.L., van der Sar, A.M., Ellett, F., Lieschke, G.J., Spaink, H.P., and Meijer, A.H. (2012) Infection of zebrafish embryos with intracellular bacterial pathogens. Journal of visualized experiments : JoVE. (61)
Dworkin, S., Darido, C., Georgy, S.R., Wilanowski, T., Srivastava, S., Ellett, F., Pase, L., Han, Y., Meng, A., Heath, J.K., Lieschke, G.J., and Jane, S.M. (2012) Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways. Development (Cambridge, England). 139(3):525-536
Ellett, F., and Lieschke, G.J. (2012) Computational quantification of fluorescent leukocyte numbers in zebrafish embryos. Methods in enzymology. 506:425-435
Lam, N.T., Currie, P.D., Lieschke, G.J., Rosenthal, N.A., and Kaye, D.M. (2012) Nerve Growth Factor Stimulates Cardiac Regeneration via Cardiomyocyte Proliferation in Experimental Heart Failure. PLoS One. 7(12):e53210
Pase, L., Nowell, C.J., and Lieschke, G.J. (2012) In vivo real-time visualization of leukocytes and intracellular hydrogen peroxide levels during a zebrafish acute inflammation assay. Methods in enzymology. 506:135-156
Wittmann, C., Chockley, P., Singh, S.K., Pase, L., Lieschke, G.J., and Grabher, C. (2012) Hydrogen peroxide in inflammation: messenger, guide, and assassin. Advances in hematology. 2012:541471
Fang, L., Green, S.R., Baek, J.S., Lee, S.H., Ellett, F., Deer, E., Lieschke, G.J., Witztum, J.L., Tsimikas, S., and Miller, Y.I. (2011) In vivo visualization and attenuation of oxidized lipid accumulation in hypercholesterolemic zebrafish. J. Clin. Invest.. 121(12):4861-9
Ellett, F., Pase, L., Hayman, J.W., Andrianopoulos, A., and Lieschke, G.J. (2011) mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish. Blood. 117(4):e49-e56
Keightley, M.C., Layton, J.E., Hayman, J.W., Heath, J.K., and Lieschke, G.J. (2011) Mediator subunit 12 is required for neutrophil development in zebrafish. PLoS One. 6(8):e23845
Pase, L., and Lieschke, G.J. (2011) Discerning Different In vivo Roles of MicroRNAs by Experimental Approaches in Zebrafish. Methods in cell biology. 104:353-378
Berger, J., Berger, S., Hall, T.E., Lieschke, G.J., and Currie, P.D. (2010) Dystrophin-deficient zebrafish feature aspects of the Duchenne muscular dystrophy pathology. Neuromuscular disorders : NMD. 20(12):826-832
Ellett, F., and Lieschke, G.J. (2010) Zebrafish as a model for vertebrate hematopoiesis. Current opinion in pharmacology. 10(5):563--570
Lieschke, G.J., and Trede, N.S. (2009) Fish immunology. Current biology : CB. 19(16):R678-R682
de Jong-Curtain, T.A., Parslow, A.C., Trotter, A.J., Hall, N.E., Verkade, H., Tabone, T., Christie, E.L., Crowhurst, M.O., Layton, J.E., Shepherd, I.T., Nixon, S.J., Parton, R.G., Zon, L.I., Stainier, D.Y., Lieschke, G.J., and Heath, J.K. (2009) Abnormal nuclear pore formation triggers apoptosis in the intestinal epithelium of elys-deficient zebrafish. Gastroenterology. 136(3):902-911
Ellett, F., Kile, B.T., and Lieschke, G.J. (2009) The role of the ETS factor erg in zebrafish vasculogenesis. Mechanisms of Development. 126(3-4):220-229
Pase, L., Layton, J.E., Kloosterman, W.P., Carradice, D., Waterhouse, P.M., and Lieschke, G.J. (2009) miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2. Blood. 113(8):1794-1804
Pase, L., and Lieschke, G.J. (2009) Validating microRNA Target Transcripts Using Zebrafish Assays. Methods in molecular biology (Clifton, N.J.). 546:227-240
Carradice, D., and Lieschke, G.J. (2008) Zebrafish in hematology: sushi or science?. Blood. 111(7):3331-3342
Aleström, P., Begemann, G., Carvan, M.J. 3rd, Cheng, K.C., Crosier, K., Crosier, P., Ekker, S., Huttenlocher, A., Kawakami, K., Kelly, G., Korzh, V., Lieschke, G., Mione, M., Neely, M.N., Neuhauss, S., and Trede, N.S. (2008) Views on four key questions about zebrafish research. Zebrafish. 5(1):9-24
Hogan, B.M., Verkade, H., Lieschke, G.J., and Heath, J.K. (2008) Manipulation of gene expression during zebrafish embryonic development using transient approaches. Methods in molecular biology (Clifton, N.J.). 469:273-300
Dworkin, S., Heath, J.K., Dejong-Curtain, T.A., Hogan, B.M., Lieschke, G.J., Malaterre, J., Ramsay, R.G., and Mantamadiotis, T. (2007) CREB activity modulates neural cell proliferation, midbrain-hindbrain organization and patterning in zebrafish. Developmental Biology. 307(1):127-141
Horsfield, J.A., Anagnostou, S.H., Hu, J.K., Cho, K.H., Geisler, R., Lieschke, G., Crosier, K.E., and Crosier, P.S. (2007) Cohesin-dependent regulation of Runx genes. Development (Cambridge, England). 134(14):2639-2649
Lieschke, G.J., and Currie, P.D. (2007) Animal models of human disease: zebrafish swim into view. Nature reviews. Genetics. 8(5):353-367
Yoong, S., O'connell, B., Soanes, A., Crowhurst, M.O., Lieschke, G.J., and Ward, A.C. (2007) Characterization of the zebrafish matrix metalloproteinase 9 gene and its developmental expression pattern. Gene expression patterns : GEP. 7(1-2):39-46
Hogan, B.M., Pase, L., Hall, N.E., and Lieschke, G.J. (2006) Characterisation of duplicate zinc finger like 2 erythroid precursor genes in zebrafish. Development genes and evolution. 216(9):523-529
Kinna, G., Kolle, G., Carter, A., Key, B., Lieschke, G.J., Perkins, A., and Little, M.H. (2006) Knockdown of zebrafish crim1 results in a bent tail phenotype with defects in somite and vascular development. Mechanisms of Development. 123(4):277-287
Hogan, B.M., Layton, J.E., Pyati, U.J., Nutt, S.L., Hayman, J.W., Varma, S., Heath, J.K., Kimelman, D., and Lieschke, G.J. (2006) Specification of the Primitive Myeloid Precursor Pool Requires Signaling through Alk8 in Zebrafish. Current biology : CB. 16(5):506-511
Hogan, B.M., Danks, J.A., Layton, J.E., Hall, N.E., Heath, J.K., and Lieschke, G.J. (2005) Duplicate zebrafish pth genes are expressed along the lateral line and in the central nervous system during embryogenesis. Endocrinology. 146(2):547-551
Onnebo, S.M., Condron, M.M., McPhee, D.O., Lieschke, G.J., and Ward, A.C. (2005) Hematopoietic perturbation in zebrafish expressing a tel-jak2a fusion. Experimental hematology. 33(2):182-188
Hogan, B.M., Hunter, M.P., Oates, A.C., Crowhurst, M.O., Hall, N.E., Heath, J.K., Prince, V.E., and Lieschke, G.J. (2004) Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm. Developmental Biology. 276(2):508-522
Mawdsley, D.J., Cooper, H.M., Hogan, B.M., Cody, S.H., Lieschke, G.J., and Heath, J.K. (2004) The Netrin receptor Neogenin is required for neural tube formation and somitogenesis in zebrafish. Developmental Biology. 269(1):302-315
Ward, A.C., McPhee, D.O., Condron, M.M., Varma, S., Cody, S.H., Onnebo, S.M., Paw, B.H., Zon, L.I., and Lieschke, G.J. (2003) The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish. Blood. 102(9):3238-3240
Lieschke, G.J., Oates, A.C., Paw, B.H., Thompson, M.A., Hall, N.E., Ward, A.C., Ho, R.K., Zon, L.I., and Layton, J.E. (2002) Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning. Developmental Biology. 246(2):274-295
Crowhurst, M.O., Layton, J.E., and Lieschke, G.J. (2002) Developmental biology of zebrafish myeloid cells. The International journal of developmental biology. 46(4):483-492
Layton, J.E., Hall, N.E., Connell, F., Varma, S., Fujiki, K., and Lieschke, G.J. (2001) Cloning and characterization of zebrafish gp130. Blood. 98(11):4200
Lieschke, G.J., Oates, A.C., Crowhurst, M.O., Ward, A.C., and Layton, J.E. (2001) Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish. Blood. 98(10):3087-3096
Lieschke, G.J. (2001) Zebrafish--an emerging genetic model for the study of cytokines and hematopoiesis in the era of functional genomics. International journal of hematology. 73(1):23-31
Liao, E.C., Paw, B.H., Peters, L.L., Zapata, A., Pratt, S.J., Do, C.P., Lieschke, G., and Zon, L.I. (2000) Hereditary spherocytosis in zebrafish riesling illustrates evolution of erythroid ß-spectrin structure, and function in red cell morphogenesis and membrane stability. Development (Cambridge, England). 127(23):5123-5132