ZFIN ID: ZDB-LAB-970409-25
Yamasu Lab
PI/Director: Yamasu, Kyo
Co-PI / Senior
Kawamura, Akinori
Contact Person: Yamasu, Kyo
Email: kyamasu@mail.saitama-u.ac.jp
URL: http://devbiol.seitai.saitama-u.ac.jp/
Address: Division of Life Science Graduate School of Science and Engineering Saitama University Shimo-Okubo 255, Sakura-ku Saitama 338-8570 JAPAN
Phone: 81-48-858-3417
Fax: 81-48-858-3698
Line Designation: sud


1. Transcriptional regulation in zebrafish embryo, with special reference to the positional information along body axes.

2. Molecular mechanism underlying the regionalization of the brain primordium during development.

Nakayama, Yukiko Graduate Student Ota, Satoshi Graduate Student

Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., Kawahara, A. (2015) Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish Sci. Rep.5:8841.
Ota, S., Hisano, Y., Ikawa, Y., Kawahara, A. (2014) Multiple genome modifications by the CRISPR/Cas9 system in zebrafish Genes Cells19(7):555-64.
Ota, S., and Kawahara, A. (2013) Zebrafish: a model vertebrate suitable for the analysis of human genetic disorders Congenit. Anom.54(1):8-11.
Hisano, Y., Ota, S., and Kawahara, A. (2013) Genome editing using artificial site-specific nucleases in zebrafish Dev. Growth Differ.56(1):26-33.
Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K. (2013) Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner Mech. Dev.130(11-12):532-52.
Hisano, Y., Ota, S., Takada, S., and Kawahara, A. (2013) Functional cooperation of spns2 and fibronectin in cardiac and lower jaw development Biol. Open2(8):789-794.
Ota, S., Hisano, Y., Muraki, M., Hoshijima, K., Dahlem, T.J., Grunwald, D.J., Okada, Y., and Kawahara, A. (2013) Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays Genes Cells18(6):450-458.
Hisano, Y., Ota, S., Arakawa, K., Muraki, M., Kono, N., Oshita, K., Sakuma, T., Tomita, M., Yamamoto, T., Okada, Y., and Kawahara, A. (2013) Quantitative assay for TALEN activity at endogenous genomic loci Biol. Open2(4):363-367.
Khan, A., Nakamoto, A., Okamoto, S., Tai, M., Nakayama, Y., Kobayashi, K., Kawamura, A., Takeda, H., and Yamasu, K. (2012) Pou2, a class V POU-type transcription factor in zebrafish, regulates dorsoventral patterning and convergent extension movement at different blastula stages Mech. Dev.129(9-12):219-235.
Khan, A., Nakamoto, A., Tai, M., Saito, S., Nakayama, Y., Kawamura, A., Takeda, H., and Yamasu, K. (2012) Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis Dev. Growth Differ.54(7):686-701.
Ota, S., Ishitani, S., Shimizu, N., Matsumoto, K., Itoh, M., and Ishitani, T. (2012) NLK positively regulates Wnt/beta-catenin signalling by phosphorylating LEF1 in neural progenitor cells EMBO J.31(8):1904-1915.
Ishioka, A., Jindo, T., Kawanabe, T., Hatta, K., Parvin, M.S., Nikaido, M., Kuroyanagi, Y., Takeda, H., and Yamasu, K. (2011) Retinoic acid-dependent establishment of positional information in the hindbrain was conserved during vertebrate evolution Dev. Biol.350(1):154-168.
Ota, S., Tonou-Fujimori, N., Nakayama, Y., Ito, Y., Kawamura, A., and Yamasu, K. (2010) FGF receptor gene expression and its regulation by FGF signaling during early zebrafish development Genesis48(12):707-716.
Ota, S., Tonou-Fujimori, N., and Yamasu, K. (2009) The roles of the FGF signal in zebrafish embryos analyzed using constitutive activation and dominant-negative suppression of different FGF receptors Mech. Dev.126(1-2):1-17.
Kawamura, A., Koshida, S., and Takada, S. (2008) Activator-to-repressor conversion of T-box transcription factors by the Ripply family of Groucho/TLE-associated mediators Mol. Cell. Biol.28(10):3236-3244.
Parvin, M.S., Okuyama, N., Inoue, F., Islam, M.E., Kawakami, A., Takeda, H., and Yamasu, K. (2008) Autoregulatory loop and retinoic acid repression regulate pou2/pou5f1 gene expression in the zebrafish embryonic brain Dev. Dyn.237(5):1373-1388.
Inoue, F., Parvin, M.S., and Yamasu, K. (2008) Transcription of fgf8 is regulated by activating and repressive cis-elements at the midbrain-hindbrain boundary in zebrafish embryos Dev. Biol.316(2):471-486.
Akanuma, T., Koshida, S., Kawamura, A., Kishimoto, Y., and Takada, S. (2007) Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation EMBO Rep.8(9):858-863.
Nikaido, M., Doi, K., Shimizu, T., Hibi, M., Kikuchi, Y., and Yamasu, K. (2007) Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal Dev. Dyn.236(2):564-571.
Islam, M.E., Kikuta, H., Inoue, F., Kanai, M., Kawakami, A., Parvin, M.S., Takeda, H., and Yamasu, K. (2006) Three enhancer regions regulate gbx2 gene expression in the isthmic region during zebrafish development Mech. Dev.123(12):907-924.
Inoue, F., Nagayoshi, S., Ota, S., Islam, M.E., Tonou-Fujimori, N., Odaira, Y., Kawakami, K., and Yamasu, K. (2006) Genomic organization, alternative splicing, and multiple regulatory regions of the zebrafish fgf8 gene Dev. Growth Differ.48(7):447-462.
Kawamura, A., Koshida, S., Hijikata, H., Ohbayashi, A., Kondoh, H., and Takada, S. (2005) Groucho-associated transcriptional repressor ripply1 is required for proper transition from the presomitic mesoderm to somites Dev. Cell9(6):735-744.
Kawamura, A., Koshida, S., Hijikata, H., Sakaguchi, T., Kondoh, H., and Takada, S. (2005) Zebrafish Hairy/Enhancer of split protein links FGF signaling to cyclic gene expression in the periodic segmentation of somites Genes Dev.19(10):1156-1161.
Komoike, Y., Kawamura, A., Shindo, N., Sato, C., Satoh, J., Shiurba, R., and Higashinakagawa, T. (2005) Zebrafish Polycomb group gene ph2alpha is required for epiboly and tailbud formation acting downstream of FGF signaling Biochem. Biophys. Res. Commun.328(4):858-866.
Kikuta, H., Kanai, M., Ito, Y., and Yamasu, K. (2003) gbx2 Homeobox gene is required for the maintenance of the isthmic region in the zebrafish embryonic brain Dev. Dyn.228(3):433-450.
Hirate, Y., Okamoto, H. and Yamasu, K. (2003) Structure of the zebrafish fasciclin I-related extracellular matrix protein (betaig-h3) and its characteristic expression during embryogenesis Gene Expr. Patterns3(3):331-336.
Tonou-Fujimori, N., Takahashi, M., Onodera, H., Kikuta, H., Koshida, S., Takeda, H., and Yamasu, K. (2002) Expression of the FGF receptor 2 gene (fgfr2) during embryogenesis in the zebrafish Danio rerio Mech. Dev.(Suppl.) 119:S173-S178.
Kawamura, A., Yokota, S., Yamada, K., Inoue, H., Inohaya, K., Yamazaki, K., Yasumasu, I., and Higashinakagawa, T. (2002) pc1 and psc1, zebrafish homologs of Drosophila Polycomb and Posterior sex combs, encode nuclear proteins capable of complex interactions Biochem. Biophys. Res. Commun.294(2):456-463.
Kawamura, A., Yamada, K., Fujimori, K., and Higashinakagawa, T. (2002) Alternative transcripts of a polyhomeotic gene homolog are expressed in distinct regions of somites during segmentation of zebrafish embryos Biochem. Biophys. Res. Commun.291(2):245-254.
Hirate, Y., Mieda, M., Harada, T., Yamasu, K., and Okamoto, H. (2001) Identification of ephrin-A3 and novel genes specific to the midbrain-MHB in embryonic zebrafish by ordered differential display Mech. Dev.107(1-2):83-96.
Sawada, A., Fritz, A., Jiang, Y., Yamamoto, A., Yamasu, K., Kuroiwa, A., Saga, Y., and Takeda, H. (2000) Zebrafish Mesp family genes, mesp-a and mesp-b are segmentally expressed in the presomitic mesoderm, and Mesp-b confers the anterior identity to the developing somites Development127(8):1691-1702.