ZFIN ID: ZDB-LAB-190716-1
Gray Lab
PI/Director: Gray, Ryan
Contact Person: Gray, Ryan
Email: ryan.gray@austin.utexas.edu
URL:
Address: 1400 Barbara Jordan Blvd. Austin, Texas 78723
Country: United States
Phone: 512-495-5052
Fax:
Line Designation: ut


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 2 genomic features


STATEMENT OF RESEARCH INTERESTS
The goal of my lab is the study of the cellular and molecular mechanisms of spine development and homeostasis using explicit animal models. We choose to utilize both mouse and zebrafish models to provide complementary approaches to study musculoskeletal development and disease. For instance, we developed a conditional mouse model of idiopathic scoliosis (IS) by removing Gpr126/Adgrg6 function in osteochondral progenitors (Human Molecular Genetics, 2015). Mechanistic studies of this mutant mouse model have begun to focus our investigation towards the identification of mechanisms of cartilage homeostasis and joint degeneration. We have also shown that zebrafish can model aspects of IS (Development Dynamics, 2014), as well as, other vertebral malformations relevant to human disease (Developmental Biology, 2014). In an effort to promote gene discovery, we also utilized the power of zebrafish genetics to identify a collection of scoliosis mutant zebrafish. Our studies in both zebrafish and mouse have begun to identify common mechanistic roles for scoliosis and hydrocephalus affecting alterations in the physiology of the cerebrospinal fluid (PLOS Genetics, 2018). We will continue to use a multi-tiered approach, combining zebrafish, mouse, and cell culture models, informed by human genomic studies, with the goal to inform the diagnosis of pediatric/musculoskeletal diseases and provide avenues for therapeutic interventions of these disorders in humans.


LAB MEMBERS
Liu, Zhaoyang Post-Doc Troutwine, Benjamin Post-Doc Konjikusic, Mia Graduate Student
Minowa, Ryoko Technical Staff Wang, Yunija


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Konjikusic, M.J., Yeetong, P., Boswell, C.W., Lee, C., Roberson, E.C., Ittiwut, R., Suphapeetiporn, K., Ciruna, B., Gurnett, C.A., Wallingford, J.B., Shotelersuk, V., Gray, R.S. (2018) Mutations in Kinesin family member 6 reveal specific role in ependymal cell ciliogenesis and human neurological development. PLoS Genetics. 14:e1007817
Haller, G., McCall, K., Jenkitkasemwong, S., Sadler, B., Antunes, L., Nikolov, M., Whittle, J., Upshaw, Z., Shin, J., Baschal, E., Cruchaga, C., Harms, M., Raggio, C., Morcuende, J.A., Giampietro, P., Miller, N.H., Wise, C., Gray, R.S., Solnica-Krezel, L., Knutson, M., Dobbs, M.B., Gurnett, C.A. (2018) A missense variant in SLC39A8 is associated with severe idiopathic scoliosis. Nature communications. 9:4171
Herbert, A.L., Fu, M.M., Drerup, C.M., Gray, R.S., Harty, B.L., Ackerman, S.D., O'Reilly-Pol, T., Johnson, S.L., Nechiporuk, A.V., Barres, B.A., Monk, K.R. (2017) Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.. Proceedings of the National Academy of Sciences of the United States of America. 114:E9153-E9162
Sanchez, N.E., Harty, B.L., O'Reilly-Pol, T., Ackerman, S.D., Herbert, A.L., Holmgren, M., Johnson, S.L., Gray, R.S., Monk, K.R. (2017) Whole Genome Sequencing-Based Mapping and Candidate Identification of Mutations from Fixed Zebrafish Tissue. G3 (Bethesda). 7(10):3415-3425
Buchan, J.G., Gray, R.S., Gansner, J.M., Alvarado, D.M., Burgert, L., Gitlin, J.D., Gurnett, C.A., Goldsmith, M.I. (2014) Kinesin family member 6 (kif6) is necessary for spine development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 243(12):1646-57
Gray, R.S., Wilm, T.P., Smith, J., Bagnat, M., Dale, R.M., Topczewski, J., Johnson, S.L., and Solnica-Krezel, L. (2014) Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations. Developmental Biology. 386(1):72-85