ZFIN Lab: Johnston Lab

ZFIN ID: ZDB-LAB-170322-1

ZFIN ID: ZDB-LAB-170322-1

Johnston Lab

PI/Director:	Johnston, Simon
Contact Person:	Drew, David
Email:	d.drew@sheffield.ac.uk
URL:	http://www.johnstonlabs.co.uk
Address:	Department of Infection, Immunity and Cardiovascular Disease, School of Medicine Bateson Centre and The Krebs Institute, University of Sheffield
Country:	United Kingdom
Phone:	+44 (0)114 2222 301
Fax:	+44 (0)114 2222 301
Line Designation:	sh

GENOMIC FEATURES ORIGINATING FROM THIS LAB

Show all 1 genomic features

STATEMENT OF RESEARCH INTERESTS

Our lab studies macrophage biology and host pathogen interactions. We are interested in normal immunity to infection and opportunistic infection of the immunocompromised. We combine in vitro immune cell and in vivo zebrafish experimental models with multidisciplinary work with engineers, physicists and mathematicians.

LAB MEMBERS

Evans, Robert Post-Doc	Fehri, Hamid Graduate Student	Gibson, Josie Graduate Student
Kamuyango, Alfred Graduate Student	Pline, Kate Graduate Student	Bojarczuk, Aleks Research Staff
Drew, David Technical Staff

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Gibson, J.F., Bojarczuk, A., Evans, R.J., Kamuyango, A.A., Hotham, R., Lagendijk, A.K., Hogan, B.M., Ingham, P.W., Renshaw, S.A., Johnston, S.A. (2022) Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection. PLoS pathogens. 18:e1010389

Gibson, J.F., Pidwill, G.R., Carnell, O.T., Surewaard, B.G.J., Shamarina, D., Sutton, J.A.F., Jeffery, C., Derré-Bobillot, A., Archambaud, C., Siggins, M.K., Pollitt, E.J.G., Johnston, S.A., Serror, P., Sriskandan, S., Renshaw, S.A., Foster, S.J. (2021) Commensal bacteria augment Staphylococcus aureus infection by inactivation of phagocyte-derived reactive oxygen species. PLoS pathogens. 17:e1009880

Sutton, J.A.F., Carnell, O.T., Lafage, L., Gray, J., Biboy, J., Gibson, J.F., Pollitt, E.J.G., Tazoll, S.C., Turnbull, W., Hajdamowicz, N.H., Salamaga, B., Pidwill, G.R., Condliffe, A.M., Renshaw, S.A., Vollmer, W., Foster, S.J. (2021) Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction. PLoS pathogens. 17:e1009468

Gibson, J.F., Prajsnar, T.K., Hill, C.J., Tooke, A.K., Serba, J.J., Tonge, R.D., Foster, S.J., Grierson, A.J., Ingham, P.W., Renshaw, S.A., Johnston, S.A. (2020) Neutrophils use selective autophagy receptor Sqstm1/p62 to target Staphylococcus aureus for degradation in vivo in zebrafish. Autophagy. 17(6):1448-1457

Prajsnar, T.K., Serba, J.J., Dekker, B.M., Gibson, J.F., Masud, S., Fleming, A., Johnston, S.A., Renshaw, S.A., Meijer, A.H. (2020) The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils. Autophagy. 17(4):888-902

Evans, R.J., Pline, K., Loynes, C.A., Needs, S., Aldrovandi, M., Tiefenbach, J., Bielska, E., Rubino, R.E., Nicol, C.J., May, R.C., Krause, H.M., O'Donnell, V.B., Renshaw, S.A., Johnston, S.A. (2019) 15-keto-prostaglandin E2 activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promote Cryptococcus neoformans growth during infection. PLoS pathogens. 15:e1007597

Duvenage, L., Walker, L.A., Bojarczuk, A., Johnston, S.A., MacCallum, D.M., Munro, C.A., Gourlay, C.W. (2019) Inhibition of Classical and Alternative Modes of Respiration in Candida albicans Leads to Cell Wall Remodeling and Increased Macrophage Recognition. mBio. 10(1):

van Leeuwen, L.M., Evans, R.J., Jim, K.K., Verboom, T., Fang, X., Bojarczuk, A., Malicki, J., Johnston, S.A., van der Sar, A.M. (2018) A transgenic zebrafish model for the in vivo study of the blood and choroid plexus brain barriers using claudin 5.. Biology Open. 7(2)

Gilbert, A.S., Seoane, P.I., Sephton-Clark, P., Bojarczuk, A., Hotham, R., Giurisato, E., Sarhan, A.R., Hillen, A., Velde, G.V., Gray, N.S., Alessi, D.R., Cunningham, D.L., Tournier, C., Johnston, S.A., May, R.C. (2017) Vomocytosis of live pathogens from macrophages is regulated by the atypical MAP kinase ERK5. Science advances. 3:e1700898

Dee, C.T., Nagaraju, R.T., Athanasiadis, E.I., Gray, C., Fernandez Del Ama, L., Johnston, S.A., Secombes, C.J., Cvejic, A., Hurlstone, A.F. (2016) CD4-Transgenic Zebrafish Reveal Tissue-Resident Th2- and Regulatory T Cell-like Populations and Diverse Mononuclear Phagocytes. Journal of immunology (Baltimore, Md. : 1950). 197(9):3520-3530

Bojarczuk, A., Miller, K.A., Hotham, R., Lewis, A., Ogryzko, N.V., Kamuyango, A.A., Frost, H., Gibson, R.H., Stillman, E., May, R.C., Renshaw, S.A., Johnston, S.A. (2016) Cryptococcus neoformans Intracellular Proliferation and Capsule Size Determines Early Macrophage Control of Infection. Scientific Reports. 6:21489

Robertson, A.L., Holmes, G.R., Bojarczuk, A.N., Burgon, J., Loynes, C.A., Chimen, M., Sawtell, A.K., Hamza, B., Willson, J., Walmsley, S.R., Anderson, S.R., Coles, M.C., Farrow, S.N., Solari, R., Jones, S., Prince, L.R., Irimia, D., Rainger, G.E., Kadirkamanathan, V., Whyte, M.K., Renshaw, S.A. (2014) A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism. Science Translational Medicine. 6:225ra29