ZFIN ID: ZDB-LAB-160721-1
Jusuf Lab
PI/Director: Jusuf, Patricia
Contact Person: Dudczig, Stefanie
Email: stefanie.dudczig@unimelb.edu.au
URL:
Address: School of Biosciences The University of Melbourne Biosciences 5/ Building 184 Parkville, Victoria 3010 Australia
Country: Australia
Phone: +61 3 8344 9519
Fax:
Line Designation: uom


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 1 genomic features


STATEMENT OF RESEARCH INTERESTS
Our group is interested in understanding the genetic networks that co-ordinate the generation of different types of neurons in the central nervous system of vertebrates. We use zebrafish as a model and focus on neural specification in the retina in the eye, which has a relatively simpler organisation compared to other CNS regions.

Zebrafish grow quickly and are optically transparent, allowing us to track individual cells in the forming embryo. By visualising gene expression with fluorescent reporter protein, we can study how timing and interactions of genes within developing progenitors influence what types of neurons they can generate. We can also assess the consequences of gene manipulations including those that mimic human disorders as well as study how and why the zebrafish are able to completely regenerate neurons and neural function after injury in adults.


LAB MEMBERS
Karamalakis, Anthony Graduate Student Krylov, Aaron Graduate Student Veen, Kellie Graduate Student
Xie, Jiaheng Graduate Student Dudczig, Stefanie Research Staff Brandli, Alice


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Xie, J., Jusuf, P.R., Bui, B.V., Goodbourn, P.T. (2019) Experience-dependent development of visual sensitivity in larval zebrafish. Scientific Reports. 9:18931
Xie, J., Goodbourn, P.T., Bui, B.V., Sztal, T.E., Jusuf, P.R. (2019) Correspondence Between Behavioral, Physiological, and Anatomical Measurements of Visual Function in Inhibitory Neuron-Ablated Zebrafish. Investigative ophthalmology & visual science. 60:4681-4690
Ehrlich, O., Karamalakis, A., Krylov, A.J., Dudczig, S., Hassell, K.L., Jusuf, P.R. (2019) Clove Oil and AQUI-S Efficacy for Zebrafish Embryo, Larva, and Adult Anesthesia. Zebrafish. 16(5):451-459
Xie, J., Jusuf, P.R., Goodbourn, P.T., Bui, B.V. (2019) Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode. Journal of visualized experiments : JoVE. (145):
Goldshmit, Y., Tang, J.K.K.Y., Siegel, A.L., Nguyen, P.D., Kaslin, J., Currie, P.D., Jusuf, P.R. (2018) Different Fgfs have distinct roles in regulating neurogenesis after spinal cord injury in zebrafish. Neural Development. 13:24
Dudczig, S., Currie, P.D., Jusuf, P.R. (2017) Developmental and adult characterization of secretagogin expressing amacrine cells in zebrafish retina. PLoS One. 12:e0185107
Ng Chi Kei, J., Currie, P.D., Jusuf, P.R. (2017) Fate bias during neural regeneration adjusts dynamically without recapitulating developmental fate progression. Neural Development. 12:12
Goodings, L., He, J., Wood, A., Harris, W.A., Currie, P.D., Jusuf, P.R. (2017) In vivo expression of Nurr1/Nr4a2a in developing retinal amacrine subtypes in zebrafish Tg(nr4a2a:eGFP) transgenics. The Journal of comparative neurology. 525(8):1962-1979
Dudczig, S., Currie, P.D., Poggi, L., Jusuf, P.R. (2017) In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences. Journal of visualized experiments : JoVE. (121)
Ng Chi Kei, J., Dudczig, S., Currie, P.D., Jusuf, P.R. (2016) Feedback from each retinal neuron population drives expression of subsequent fate determinant genes without influencing the cell cycle exit timing. The Journal of comparative neurology. 524(13):2553-66
Boije, H., Rulands, S., Dudczig, S., Simons, B.D., Harris, W.A. (2015) The Independent Probabilistic Firing of Transcription Factors: A Paradigm for Clonal Variability in the Zebrafish Retina. Developmental Cell. 34(5):532-43
Ng, J., Currie, P., Jusuf, P. R. (2014) The Regenerative Potential of the Vertebrate Retina: Lessons from the Zebrafish. Regenerative Biology of the Eye. :49-82
Jusuf, P., Harris, W.A., and Poggi, L. (2013) Preparation of transgenic zebrafish embryos for imaging the developing retina. Cold Spring Harbor protocols. 2013(3):pdb.prot073536
Jusuf, P., Harris, W.A., and Poggi, L. (2013) Imaging retinal progenitor lineages in developing zebrafish embryos. Cold Spring Harbor protocols. 2013(3):pdb.prot073544
Kizil, C., Kyritsis, N., Dudczig, S., Kroehne, V., Freudenreich, D., Kaslin, J., and Brand, M. (2012) Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3. Developmental Cell. 23(6):1230-1237
Jusuf, P.R., Albadri, S., Paolini, A., Currie, P.D., Argenton, F., Higashijima, S., Harris, W.A., and Poggi, L. (2012) Biasing Amacrine Subtypes in the Atoh7 Lineage through Expression of Barhl2. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(40):13929-13944
Kizil, C., Dudczig, S., Kyritsis, N., Machate, A., Blaesche, J., Kroehne, V., and Brand, M. (2012) The chemokine receptor cxcr5 regulates the regenerative neurogenesis response in the adult zebrafish brain. Neural Development. 7(1):27
Goldshmit, Y., Sztal, T.E., Jusuf, P.R., Hall, T.E., Nguyen-Chi, M., and Currie, P.D. (2012) Fgf-dependent glial cell bridges facilitate spinal cord regeneration in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(22):7477-7492
Jusuf, P.R., Almeida, A.D., Randlett, O., Joubin, K., Poggi, L., and Harris, W.A. (2011) Origin and determination of inhibitory cell lineages in the vertebrate retina. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(7):2549-2562
Jusuf, P.R., and Harris, W.A. (2009) Ptf1a is expressed transiently in all types of amacrine cells in the embryonic zebrafish retina. Neural Development. 4:34
Vitorino, M., Jusuf, P.R., Maurus, D., Yukiko, Y., Higashijima, S.I., and Harris, W.A. (2009) Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development. 4:14