ZFIN ID: ZDB-LAB-150417-1
Wei Ge Lab
PI/Director: Ge, Wei
Contact Person: Ai, Nana
Email: ainadiandian@126.com
URL: http://fhs.umac.mo/staff/academic-staff/wei-ge/
Address: Reproductive Biology Laboratory, Open Research Base (N22), University of Macau, Avenida da Universidade, Taipa, Macau, China
Country: China
Phone: +853-88222526
Fax: +853-88222314
Line Designation: umo


GENOMIC FEATURES ORIGINATING FROM THIS LAB
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STATEMENT OF RESEARCH INTERESTS
My research focuses on functional analysis of the brain-pituitary-ovary axis using zebrafish as the model. Our current interests include: (1) endocrine regulation of reproduction by the reproductive and growth axes; (2) intra-follicular paracrine/autocrine communication network in the ovary; (3) impacts of environmental endocrine-disrupting chemicals (EDCs) on reproduction and public health; and (4) zebrafish as a model for aging, human diseases and drug discovery. Our research involves using a variety of techniques in molecular and cell biology, biochemistry, physiology and genetics, including the newly-emerged genome-editing technologies, i.e., TALEN and CRISPR/Cas9.


LAB MEMBERS
Chen, Weiting Post-Doc Lu, Huijie Post-Doc Zhang, Zhiwei Post-Doc
Hu, Zhe Graduate Student Qin, Mingming Graduate Student Zhang, Yibo Graduate Student
Zhu, Bo Graduate Student Choi, Cherry Research Staff Fan, Yuqin Research Staff
Ng, Pheobe Research Staff Ai, Nana Jiang, Yingda


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Bian, C., Chen, W., Ruan, Z., Hu, Z., Huang, Y., Lv, Y., Xu, T., Li, J., Shi, Q., Ge, W. (2020) Genome and Transcriptome Sequencing of casper and roy Zebrafish Mutants Provides Novel Genetic Clues for Iridophore Loss. International Journal of Molecular Sciences. 21(7):
Yu, J., Zhong, B., Jin, L., Hou, Y., Ai, N., Ge, W., Li, L., Liu, S., Lu, J.J., Chen, X. (2020) 2-Methoxy-6-acetyl-7-methyljuglone (MAM) induced programmed necrosis in glioblastoma by targeting NAD(P)H: Quinone oxidoreductase 1 (NQO1). Free radical biology & medicine. 152:336-347
Zhu, X., Ding, S., Li, H., Zhang, Z., Xu, L., Wu, J., Wang, X., Zou, Y., Yang, X., Ge, J. (2020) Disruption of histamine/H1R signaling pathway represses cardiac differentiation and maturation of human induced pluripotent stem cells. Stem Cell Research & Therapy. 11:27
Li, J., Ge, W. (2020) Zebrafish as a model for studying ovarian development: Recent advances from targeted gene knockout studies. Molecular and Cellular Endocrinology. 507:110778
Jia, H., Song, Y., Huang, B., Ge, W., Luo, K.Q. (2020) Engineered Sensor Zebrafish for Fast Detection and Real-Time Tracking of Apoptosis at Single-Cell Resolution in Live Animals. ACS sensors. 5(3):823-830
Wu, K., Song, W., Zhang, Z., Ge, W. (2020) Disruption of dmrt1 rescues the all-male phenotype of cyp19a1a mutant in zebrafish - A Novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis. Development (Cambridge, England). 147(4):
Song, W., Lu, H., Wu, K., Zhang, Z., Shuk-Wa Lau, E., Ge, W. (2019) Genetic evidence for estrogenicity of bisphenol A in zebrafish gonadal differentiation and its signalling mechanism. Journal of hazardous materials. 386:121886
Li, J., Cui, Y., Bi, C., Feng, S., Yu, F., Yuan, E., Xu, S., Hu, Z., Sun, Q., Wei, D., Yoon, J. (2019) Oligo(ethylene glycol)-Functionalized Ratiometric Fluorescent Probe for the Detection of Hydrazine in Vitro and in Vivo. Analytical chemistry. 91(11):7360-7365
Hu, Z., Ai, N., Chen, W., Wong, Q.W., Ge, W. (2019) Loss of growth hormone gene (gh1) in zebrafish arrests folliculogenesis in females and delays spermatogenesis in males. Endocrinology. 160(3):568-586
Zhu, B., Pardeshi, L., Chen, Y., Ge, W. (2018) Transcriptomic Analysis for Differentially Expressed Genes in Ovarian Follicle Activation in the Zebrafish. Frontiers in endocrinology. 9:593
Qin, M., Zhang, Z., Song, W., Wong, Q.W., Chen, W., Shirgaonkar, N., Ge, W. (2018) Roles of Figla/figla in Juvenile Ovary Development and Follicle Formation during Zebrafish Gonadogenesis. Endocrinology. 159(11):3699-3722
Lyu, J., Yang, E.J., Head, S.A., Ai, N., Zhang, B., Wu, C., Li, R.J., Liu, Y., Chakravarty, H., Zhang, S., Tam, K.Y., Dang, Y., Kwon, H.J., Ge, W., Liu, J.O., Shim, J.S. (2018) Astemizole Inhibits mTOR Signaling and Angiogenesis by Blocking Cholesterol Trafficking. International journal of biological sciences. 14:1175-1185
Zhang, Y., Zhang, Z., Ge, W. (2018) An Efficient Platform for Generating Somatic Point Mutations with Germline Transmission in the Zebrafish by CRISPR/Cas9-mediated Gene Editing. The Journal of biological chemistry. 293(17):6611-6622
Ai, N., Chong, C.M., Chen, W., Hu, Z., Su, H., Chen, G., Lei Wong, Q.W., Ge, W. (2018) Ponatinib exerts anti-angiogenic effects in the zebrafish and human umbilical vein endothelial cells via blocking VEGFR signaling pathway. Oncotarget. 9:31958-31970
Wong, Q.W., Sun, M.A., Lau, S.W., Parsania, C., Zhou, S., Zhong, S., Ge, W. (2017) Identification and characterization of a specific 13-miRNA expression signature during follicle activation in the zebrafish ovary. Biology of reproduction. 98(1):42-53
Lyu, J., Yang, E.J., Head, S.A., Ai, N., Zhang, B., Wu, C., Li, R.J., Liu, Y., Yang, C., Dang, Y., Kwon, H.J., Ge, W., Liu, J.O., Shim, J.S. (2017) Pharmacological blockade of cholesterol trafficking by cepharanthine in endothelial cells suppresses angiogenesis and tumor growth. Cancer letters. 409:91-103
Chen, W., Lau, S.W., Fan, Y., Wu, R.S.S., Ge, W. (2017) Juvenile exposure to bisphenol A promotes ovarian differentiation but suppresses its growth - Potential involvement of pituitary follicle-stimulating hormone. Aquatic toxicology (Amsterdam, Netherlands). 193:111-121
Lu, H., Cui, Y., Jiang, L., Ge, W. (2017) Functional analysis of nuclear estrogen receptors (nERs) in zebrafish reproduction by genome editing approach. Endocrinology. 158(7):2292-2308
Liu, K.C., Lau, S.W., Ge, W. (2017) Spatiotemporal Expression Analysis of Nuclear Estrogen Receptors in the Zebrafish Ovary and Their Regulation in Vitro by Endocrine hormones and Paracrine Factors. General and comparative endocrinology. 246:218-225
Chen, W., Liu, L., Ge, W. (2017) Expression analysis of growth differentiation factor 9 (Gdf9/gdf9), anti-müllerian hormone (Amh/amh) and aromatase (Cyp19a1a/cyp19a1a) during gonadal differentiation of the zebrafish, Danio rerio†. Biology of reproduction. 96:401-413
Lau, E.S., Zhang, Z., Qin, M., Ge, W. (2016) Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation. Scientific Reports. 6:37357
Huang, N., Guo, H., Qi, W., Zhang, Z., Rong, J., Yuan, Z., Ge, W., Jiang, H., Xi, L. (2016) Whole-body multispectral photoacoustic imaging of adult zebrafish. Biomedical Optics Express. 7:3543-3550
Wang, C., Liu, D., Chen, W., Ge, W., Hong, W., Zhu, Y., Chen, S.X. (2016) Progestin increases the expression of gonadotropins in pituitaries of male Zebrafish. The Journal of endocrinology. 230(1):143-56
Zhou, R., Yu, S.M., Ge, W. (2016) Expression and Functional Characterization of Intrafollicular GH-IGF System in the Zebrafish Ovary. General and comparative endocrinology. 232:32-42
Huang, W., Yu, X., Liang, N., Ge, W., Kwok, H.F., Lau, C.B., Li, Y., Chung, H.Y. (2016) Anti-angiogenic Activity and Mechanism of Sesquiterpene Lactones from Centipeda minima. Natural Product Communications. 11:435-8
Zhang, Z., Lau, S.W., Zhang, L., Ge, W. (2015) Disruption of Zebrafish Follicle-stimulating Hormone Receptor (fshr) but Not Luteinizing Hormone Receptor (lhcgr) Gene by TALEN Leads to Failed Follicle Activation in Females Followed by Sexual Reversal to Males. Endocrinology. 156(10):3747-62
Zhang, J., Ge, W., Yuan, Z. (2015) In vivo three-dimensional characterization of the adult zebrafish brain using a 1325 nm spectral-domain optical coherence tomography system with the 27 frame/s video rate. Biomedical Optics Express. 6:3932-40
Yao, K., Ge, W. (2015) Differential regulation of Kit Ligand A (kitlga) expression in the zebrafish ovarian follicle cells - evidence for the existence of a cyclic adenosine 3', 5' monophosphate-mediated binary regulatory system during folliculogenesis. Molecular and Cellular Endocrinology. 402:21-31
Zhang, Z., Zhu, B., Ge, W. (2015) Genetic Analysis of Zebrafish Gonadotropin (FSH and LH) Functions by TALEN-mediated Gene Disruption. Molecular endocrinology (Baltimore, Md.). 29(1):76-98
Yao, K., Lau, S.W., and Ge, W. (2014) Differential Regulation of Kit Ligand A Expression in the Ovary by IGF-I via Different Pathways. Molecular endocrinology (Baltimore, Md.). 28(1):138-150
Liu, K.C., Wu, R.S., and Ge, W. (2013) Luteinizing hormone receptor (lhcgr) as a marker gene for characterizing estrogenic endocrine-disrupting chemicals in zebrafish ovarian follicle cells. General and comparative endocrinology. 192:89-94
Li, C.W., and Ge, W. (2013) Regulation of the Activin-Inhibin-Follistatin System by Bone Morphogenetic Proteins in the Zebrafish Ovary. Biology of reproduction. 89(3):55
Yu, X., Tong, Y., Han, X.Q., Kwok, H.F., Yue, G.G., Lau, C.B., and Ge, W. (2013) Anti-Angiogenic Activity of Herba Epimedii on Zebrafish Embryos In Vivo and HUVECs In Vitro. Phytotherapy research : PTR. 27(9):1368-75
Chung, C.K., and Ge, W. (2013) Human chorionic gonadotropin (hCG) induces MAPK3/1 phosphorylation in the zebrafish ovarian follicle cells independent of EGF/EGFR pathway. General and comparative endocrinology. 188:251-7
Liu, K.C., and Ge, W. (2013) Evidence for Gating Roles of Protein Kinase A and Protein Kinase C in Estradiol-Induced Luteinizing Hormone Receptor (lhcgr) Expression in Zebrafish Ovarian Follicle Cells. PLoS One. 8(5):e62524
Chen, W., and Ge, W. (2013) Gonadal differentiation and puberty onset in the zebrafish: Evidence for the dependence of puberty onset on body growth but not age in females. Molecular reproduction and development. 80(5):384-92
Liu, K.C., and Ge, W. (2013) Differential regulation of gonadotropin receptors (fshr and lhcgr) by epidermal growth factor (EGF) in the zebrafish ovary. General and comparative endocrinology. 181:288-294
Yao, K., and Ge, W. (2013) Spatial distribution and receptor specificity of zebrafish kit system - evidence for a kit-mediated bi-directional communication system in the preovulatory ovarian follicle. PLoS One. 8(2):e56192
He, Z.H., Yue, G.G., Lau, C.B., Ge, W., and But, P.P. (2012) Antiangiogenic Effects and Mechanisms of trans-Ethyl p-Methoxycinnamate from Kaempferia galanga L. Journal of Agricultural and Food Chemistry. 60(45):11309-11317
Chung, C.K., and Ge, W. (2012) Epidermal growth factor differentially regulates activin subunits in the zebrafish ovarian follicle cells via diverse signaling pathways. Molecular and Cellular Endocrinology. 361(1-2):133-142
Li, C.W., Zhou, R., and Ge, W. (2012) Differential regulation of gonadotropin receptors by bone morphogenetic proteins in the zebrafish ovary. General and comparative endocrinology. 176(3):420-425
Chen, W., and Ge, W. (2012) Ontogenic Expression Profiles of Gonadotropins (fshb and lhb) and Growth Hormone (gh) During Sexual Differentiation and Puberty Onset in Female Zebrafish. Biology of reproduction. 86(3):73
Yu, X., Tong, Y., Kwok, F.H.F., Sze, S.C.W., Zhong, L., Lau, C.B.S., and Ge, W. (2012) Anti-angiogenic activity of Erxian Decoction, a traditional Chinese herbal formula, in zebrafish. Biological & pharmaceutical bulletin. 35(12):2119-2127
Li, C.W., and Ge, W. (2011) Spatiotemporal Expression of Bone Morphogenetic Protein Family Ligands and Receptors in the Zebrafish Ovary: A Potential Paracrine-Signaling Mechanism for Oocyte-Follicle Cell Communication. Biology of reproduction. 85(5):977-86
Liu, K.C., Lin, S.W., and Ge, W. (2011) Differential Regulation of Gonadotropin Receptors (fshr and lhcgr) by Estradiol in the Zebrafish Ovary Involves Nuclear Estrogen Receptors That Are Likely Located on the Plasma Membrane. Endocrinology. 152(11):4418-30
Zhou, R., Tsang, A.H., Lau, S.W., and Ge, W. (2011) Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) and Its Receptors in the Zebrafish Ovary: Potential Evidence for Dual Roles of PACAP in Controlling Final Oocyte Maturation. Biology of reproduction. 85(3):615-25
He, Z.H., Zhou, R., He, M.F., Lau, C.B., Yue, G.G., Ge, W., and But, P.P. (2011) Anti-angiogenic effect and mechanism of rhein from Rhizoma Rhei. Phytomedicine : international journal of phytotherapy and phytopharmacology. 18(6):470-478
He, Z.H., Ge, W., Yue, G.G., Lau, C.B., He, M.F., and But, P.P. (2010) Anti-angiogenic effects of the fruit of Alpinia oxyphylla. Journal of ethnopharmacology. 132(2):443-449
Tse, A.C., and Ge, W. (2010) Spatial localization of EGF family ligands and receptors in the zebrafish ovarian follicle and their expression profiles during folliculogenesis. General and comparative endocrinology. 167(3):397-407
Yao, K., and Ge, W. (2010) Kit system in the zebrafish ovary: evidence for functional divergence of two isoforms of kit (kita and kitb) and kit ligand (kitlga and kitlgb) during folliculogenesis. Biology of reproduction. 82(6):1216-1226
Yu, X., Lin, S.W., Kobayashi, M., and Ge, W. (2010) Expression of recombinant zebrafish follicle-stimulating hormone (FSH) in methylotropic yeast Pichia pastoris. Fish physiology and biochemistry. 36(2):273-281
Wang, Y., Li, J., Yan Kwok, A.H., Ge, W., and Leung, F.C. (2010) A Novel Prolactin-like Protein (PRL-L) Gene in Chickens and Zebrafish: Cloning and Characterization of Its Tissue Expression. General and comparative endocrinology. 166(1):200-210
Poon, S.K., So, W.K., Yu, X., Liu, L., and Ge, W. (2009) Characterization of Inhibin {alpha} Subunit (inha) in the Zebrafish - Evidence for a Potential Feedback Loop between the Pituitary and Ovary. Reproduction (Cambridge, England). 138(4):709-719
Tse, A.C., and Ge, W. (2009) Differential regulation of betacellulin and heparin-binding EGF-like growth factor in cultured zebrafish ovarian follicle cells by EGF family ligands. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 153(1):13-17
Lin, S.W., and Ge, W. (2009) Differential regulation of gonadotropins (FSH and LH) and growth hormone (GH) by neuroendocrine, endocrine, and paracrine factors in the zebrafish—An in vitro approach. General and comparative endocrinology. 160(2):183-193
He, M.F., Liu, L., Ge, W., Shaw, P.C., Jiang, R., Wu, L.W., and But, P.P. (2009) Antiangiogenic activity of Tripterygium wilfordii and its terpenoids. Journal of ethnopharmacology. 121(1):61-68
Liu, L., and Ge, W. (2007) Growth Differentiation Factor 9 and Its Spatiotemporal Expression and Regulation in the Zebrafish Ovary. Biology of reproduction. 76(2):294-302
Ge, W. (2005) Gonadotropins and their paracrine signaling network in the zebrafish ovary. Fish physiology and biochemistry. 31(2-3):209-214
Ge, W. (2005) Intrafollicular paracrine communication in the zebrafish ovary: The state of the art of an emerging model for the study of vertebrate folliculogenesis. Molecular and Cellular Endocrinology. 237(1-2):1-10
Kwok, H.F., So, W.K., Wang, Y., and Ge, W. (2005) Zebrafish Gonadotropins and Their Receptors: I. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Receptors - Evidence for Their Distinct Functions in Follicle Development. Biology of reproduction. 72(6):1370-1381
So, W.K., Kwok, H.F., and Ge, W. (2005) Zebrafish Gonadotropins and Their Receptors: II. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Subunits - Their Spatial-Temporal Expression Patterns and Receptor Specificity. Biology of reproduction. 72(6):1382-1396
Wang, Y., and Ge, W. (2004) Cloning of Epidermal Growth Factor (EGF) and EGF Receptor from the Zebrafish Ovary: Evidence for EGF as a Potential Paracrine Factor from the Oocyte to Regulate Activin/Follistatin System in the Follicle Cells. Biology of reproduction. 71(3):749-760
Pang, Y., Ge, W. (2002) Epidermal growth factor and TGFalpha promote zebrafish oocyte maturation in vitro: potential role of the ovarian activin regulatory system. Endocrinology. 143(1):47-54
Ge, W. (2000) Roles of the activin regulatory system in fish reproduction. Canadian journal of physiology and pharmacology. 78(12):1077-1085